Key features and details
- Rabbit polyclonal to p130
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p130 antibody
See all p130 primary antibodies
DescriptionRabbit polyclonal to p130
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: MOLT4 whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 128 kDa.
Recommended for human samples only.
FunctionKey regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
Developmental stageG0-restricted expression.
modificationsDuring G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
- Information by UniProt
- 130 kDa retinoblastoma associated protein antibody
- 130 kDa retinoblastoma-associated protein antibody
- IB4 antibody
All lanes : Anti-p130 antibody (ab235275) at 1/1000 dilution
Lane 1 : MOLT4 whole cell lysate at 1 µg
Lane 2 : MOLT4 whole cell lysate at 10 µg
Lane 3 : Human eye tissue at 1 µg
Lane 4 : Human eye tissue at 10 µg
All lanes : Goat anti-rabbit IgG peroxidase conjugated at 1/10000 dilution
Predicted band size: 128 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
Human Protein Atlas: Cell line RNA database 22.9 NX = MOLT4.
IP experiment was performed but did not successfully pull-down p130 in MOLT4 lysate. Antibody is not recommended for IP under these conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235275 has not yet been referenced specifically in any publications.