Overview

  • Product name

  • Description

    Rabbit polyclonal to p130
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: MOLT4 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab235275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 128 kDa.

Recommended for human samples only.

Target

  • Function

    Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
  • Sequence similarities

    Belongs to the retinoblastoma protein (RB) family.
  • Developmental stage

    G0-restricted expression.
  • Post-translational
    modifications

    During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 130 kDa retinoblastoma associated protein antibody
    • 130 kDa retinoblastoma-associated protein antibody
    • IB4 antibody
    • p130 antibody
    • PPAR-alpha-interacting complex protein 128 antibody
    • PRB2 antibody
    • Rb2 antibody
    • Rbl2 antibody
    • RBL2_HUMAN antibody
    • RBR 2 antibody
    • RBR-2 antibody
    • RBR2 antibody
    • Retinoblastoma like 2 antibody
    • Retinoblastoma-like protein 2 antibody
    • Retinoblastoma-related protein 2 antibody
    • RIC128 antibody
    see all

Images

  • All lanes : Anti-p130 antibody (ab235275) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate at 1 µg
    Lane 2 : MOLT4 whole cell lysate at 10 µg
    Lane 3 : Human eye tissue at 1 µg
    Lane 4 : Human eye tissue at 10 µg

    Secondary
    All lanes : Goat anti-rabbit IgG peroxidase conjugated at 1/10000 dilution

    Predicted band size: 128 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Human Protein Atlas:  Cell line RNA database 22.9 NX = MOLT4.
     
    IP experiment was performed but did not successfully pull-down p130 in MOLT4 lysate. Antibody is not recommended for IP under these conditions.

References

ab235275 has not yet been referenced specifically in any publications.

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