Recombinant
RabMAb

Recombinant Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)

Overview

  • Product name
    Anti-p130 antibody [EP2141Y] - BSA and Azide free
    See all p130 primary antibodies
  • Description
    Rabbit monoclonal [EP2141Y] to p130 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human p130 aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.

  • General notes

    ab247453 is a PBS-only buffer format of ab76234. Please refer to ab76234 for recommended dilutions, protocols, and image data.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
    • Sequence similarities
      Belongs to the retinoblastoma protein (RB) family.
    • Developmental stage
      G0-restricted expression.
    • Post-translational
      modifications
      During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa retinoblastoma associated protein antibody
      • 130 kDa retinoblastoma-associated protein antibody
      • IB4 antibody
      • p130 antibody
      • PPAR-alpha-interacting complex protein 128 antibody
      • PRB2 antibody
      • Rb2 antibody
      • Rbl2 antibody
      • RBL2_HUMAN antibody
      • RBR 2 antibody
      • RBR-2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma-like protein 2 antibody
      • Retinoblastoma-related protein 2 antibody
      • RIC128 antibody
      see all

    References

    ab247453 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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