• Product name

    Anti-p130 (phospho S672) antibody [EP2126Y]
    See all p130 primary antibodies
  • Description

    Rabbit monoclonal [EP2126Y] to p130 (phospho S672)
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human p130 (phospho S672). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899), treated or untreated with AP.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.



Our Abpromise guarantee covers the use of ab76255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
    • Sequence similarities

      Belongs to the retinoblastoma protein (RB) family.
    • Developmental stage

      G0-restricted expression.
    • Post-translational

      During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • 130 kDa retinoblastoma associated protein antibody
      • 130 kDa retinoblastoma-associated protein antibody
      • IB4 antibody
      • p130 antibody
      • PPAR-alpha-interacting complex protein 128 antibody
      • PRB2 antibody
      • Rb2 antibody
      • Rbl2 antibody
      • RBL2_HUMAN antibody
      • RBR 2 antibody
      • RBR-2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma-like protein 2 antibody
      • Retinoblastoma-related protein 2 antibody
      • RIC128 antibody
      see all


    • All lanes : Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255) at 1/10000 dilution

      Lane 1 : Jurkat cell lysates, treated with AP
      Lane 2 : Jurkat cell lysates, untreated

      Lysates/proteins at 10 µg/ml per lane.

      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 128 kDa
      Observed band size: 128 kDa


    This product has been referenced in:

    • Álvaro-Blanco J  et al. MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter. Nucleic Acids Res 45:9960-9975 (2017). Read more (PubMed: 28973440) »
    • Iwahori S  et al. Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem 292:6583-6599 (2017). Read more (PubMed: 28289097) »
    See all 3 Publications for this product

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