Recombinant Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986) - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free
See all p130 primary antibodies -
Description
Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251534 is the carrier-free version of ab211928.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR2389(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. -
Sequence similarities
Belongs to the retinoblastoma protein (RB) family. -
Developmental stage
G0-restricted expression. -
Post-translational
modificationsDuring G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5934 Human
- Entrez Gene: 19651 Mouse
- Omim: 180203 Human
- SwissProt: Q08999 Human
- SwissProt: Q64700 Mouse
- Unigene: 513609 Human
- Unigene: 235580 Mouse
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Alternative names
- 130 kDa retinoblastoma associated protein antibody
- 130 kDa retinoblastoma-associated protein antibody
- IB4 antibody
see all
Images
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All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution
Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with phosphatase on the membrane
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 15 secondsThis data was developed using ab211928, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 2% BSA.
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All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with phosphatase on the membrane
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 30 secondsThis data was developed using ab211928, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution
Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lanes 2-3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 30 secondsThis data was developed using ab211928, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab211928, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p130 (phospho T986) with ab211928 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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This data was developed using ab211928, the same antibody clone in a different buffer formulation.p130 (phospho T986) was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab211928 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab211928 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution Lane 1: NIH/3T3 whole cell lysate 10µg (Input). Lane 2: ab211928 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211928 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251534 has not yet been referenced specifically in any publications.