Key features and details
- Rabbit polyclonal to p15 INK4b
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p15 INK4b antibody
See all p15 INK4b primary antibodies
DescriptionRabbit polyclonal to p15 INK4b
SpecificityDetects endogenous levels of total p15 INK protein
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from human p15 INK
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53034 was purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
Our Abpromise guarantee covers the use of ab53034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
|IHC-P||Use a concentration of 2 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionInteracts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
Sequence similaritiesBelongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats.
- Information by UniProt
- CDK inhibitory protein antibody
- CDK4B Inhibitor antibody
- Cdkn2b antibody
All lanes : Anti-p15 INK4b antibody (ab53034) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with peptide
Predicted band size: 15 kDa
Observed band size: 15 kDa
ab53034 (2µg/ml) staining p15 INK4b in human colon carcinoma.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunofluorescence analysis of HeLa cells, using Anti-p15 INK4b antibody (ab53034). The picture on the right is blocked with the synthesized peptide.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab53034 has been referenced in 26 publications.
- Prikrylova T et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). PubMed: 31363131
- Weng X et al. Cyclin-dependent kinase inhibitor 2B gene is associated with the sensitivity of hepatoma cells to Sorafenib. Onco Targets Ther 12:5025-5036 (2019). PubMed: 31388306
- Chen J et al. Downregulation of MGMT promotes proliferation of intrahepatic cholangiocarcinoma by regulating p21. Clin Transl Oncol N/A:N/A (2019). PubMed: 31264147
- Wang F et al. lncRNA CADM1-AS1 inhibits cell-cycle progression and invasion via PTEN/AKT/GSK-3ß axis in hepatocellular carcinoma. Cancer Manag Res 11:3813-3828 (2019). PubMed: 31118799
- Huang Q et al. Effect of baicalin on proliferation and apoptosis in pancreatic cancer cells. Am J Transl Res 11:5645-5654 (2019). PubMed: 31632536