Anti-p15 INK4b antibody (ab53034)
Key features and details
- Rabbit polyclonal to p15 INK4b
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p15 INK4b antibody
See all p15 INK4b primary antibodies -
Description
Rabbit polyclonal to p15 INK4b -
Host species
Rabbit -
Specificity
Detects endogenous levels of total p15 INK protein.
There is 91.8% identity to CDKN2A (P42771) based on the immunogen sequence range.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide derived from human p15 INK
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53034 was purified from rabbit antiserum by affinity chromatography using epitope specific immunogen -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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| IHC-P |
Use a concentration of 2 µg/ml.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
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IHC-P
Use a concentration of 2 µg/ml. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest. -
Sequence similarities
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats. - Information by UniProt
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Database links
- Entrez Gene: 1030 Human
- Entrez Gene: 12579 Mouse
- Entrez Gene: 25164 Rat
- Omim: 600431 Human
- SwissProt: P42772 Human
- SwissProt: P55271 Mouse
- SwissProt: P55272 Rat
- Unigene: 72901 Human
see all -
Alternative names
- CDK inhibitory protein antibody
- CDK4B Inhibitor antibody
- Cdkn2b antibody
see all
Images
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Western blot - Anti-p15 INK4b antibody (ab53034)All lanes : Anti-p15 INK4b antibody (ab53034) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with peptide
Predicted band size: 15 kDa
Observed band size: 15 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p15 INK4b antibody (ab53034)ab53034 (2µg/ml) staining p15 INK4b in human colon carcinoma.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-p15 INK4b antibody (ab53034)Immunofluorescence analysis of HeLa cells, using Anti-p15 INK4b antibody (ab53034). The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p15 INK4b antibody (ab53034)Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab53034 has been referenced in 33 publications.
- Yang L et al. PRMT5 functionally associates with EZH2 to promote colorectal cancer progression through epigenetically repressing CDKN2B expression. Theranostics 11:3742-3759 (2021). PubMed: 33664859
- Li Y et al. Stabilization of p18 by deubiquitylase CYLD is pivotal for cell cycle progression and viral replication. NPJ Precis Oncol 5:14 (2021). PubMed: 33654169
- Cheng XS et al. Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice. J Cell Mol Med 24:8391-8404 (2020). PubMed: 32614521
- Kawasaki K et al. FAM111B enhances proliferation of KRAS-driven lung adenocarcinoma by degrading p16. Cancer Sci 111:2635-2646 (2020). PubMed: 32418298
- Jiang L et al. Puromycin aminonucleoside-induced podocyte injury is ameliorated by the Smad3 inhibitor SIS3. FEBS Open Bio 10:1601-1611 (2020). PubMed: 32583562