Key features and details
- Rabbit polyclonal to p150 CAF1/CAF
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p150 CAF1/CAF antibody
See all p150 CAF1/CAF primary antibodies
DescriptionRabbit polyclonal to p150 CAF1/CAF
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human p150 CAF1/CAF aa 500-550. (AAA76736.1)
- Human colon, colonic lymphoid aggregate and colon carcinoma tissue
This product was previously labelled as p150 CAF1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.|
FunctionCore component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Sequence similaritiesBelongs to the CHAF1A family.
Developmental stageActive complex is found in G1, S and G2 phases.
DomainContains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. DNA replication foci.
- Information by UniProt
- CAF 1 antibody
- CAF 1 subunit A antibody
- CAF antibody
ab101286 has not yet been referenced specifically in any publications.