Overview

  • Product name

    Anti-p150 CAF1/CAF antibody
    See all p150 CAF1/CAF primary antibodies
  • Description

    Rabbit polyclonal to p150 CAF1/CAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human p150 CAF1/CAF aa 500-550. The exact sequence is proprietary. Q13111
    Database link: AAA76736.1

  • Positive control

    • IHC-P: Human colon carcinoma tissue. WB: HeLa whole cell lysate (ab150035). IP: HeLa whole cell lysate (ab150035).
  • General notes

     This product was previously labelled as p150 CAF1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab224824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 107 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities

    Belongs to the CHAF1A family.
  • Developmental stage

    Active complex is found in G1, S and G2 phases.
  • Domain

    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. DNA replication foci.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAF 1 antibody
    • CAF 1 subunit A antibody
    • CAF antibody
    • CAF I 150 kDa subunit antibody
    • CAF I p150 antibody
    • CAF-1 subunit A antibody
    • CAF-I 150 kDa subunit antibody
    • CAF-I p150 antibody
    • CAF1 antibody
    • CAF1A_HUMAN antibody
    • CAF1B antibody
    • CAF1P150 antibody
    • CHAF1A antibody
    • Chromatin assembly factor 1 subunit A antibody
    • Chromatin Assembly Factor 1 Subunit A p150 antibody
    • Chromatin assembly factor I (150 kDa) antibody
    • Chromatin assembly factor I 150 kDa antibody
    • Chromatin assembly factor I p150 subunit antibody
    • DCAF1 antibody
    • hp150 antibody
    • MGC71229 antibody
    • P150 antibody
    see all

Images

  • All lanes : Anti-p150 CAF1/CAF antibody (ab224824) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 107 kDa


    Exposure time: 3 minutes
  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for p150 CAF1/CAF using ab224824 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

  • p150 CAF1/CAF was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224824 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224824 at 1 µg/ml.

    Lane 1: ab224824 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.


    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab224824 has not yet been referenced specifically in any publications.

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