Recombinant Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
![Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)](https://www.abcam.com/ps/products/126/ab126625/Images/ab126625-140624-anti-p150-caf1-antibody-epr55762-western-blot.jpg "Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5576(2)] to p150 CAF1/CAF
- Suitable for: WB, IP, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-p150 CAF1/CAF antibody [EPR5576(2)]
See all p150 CAF1/CAF primary antibodies -
Description
Rabbit monoclonal [EPR5576(2)] to p150 CAF1/CAF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human p150 CAF1/CAF aa 100-200. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa, HeLa treated with hydroxyurea and K562 whole cell lysates; Human colon and tonsil tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as p150 CAF1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5576(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 107 kDa).
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| IP |
1/10 - 1/100.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt |
1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 107 kDa). |
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IP
1/10 - 1/100. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt
1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. -
Sequence similarities
Belongs to the CHAF1A family. -
Developmental stage
Active complex is found in G1, S and G2 phases. -
Domain
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. DNA replication foci. - Information by UniProt
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Database links
- Entrez Gene: 10036 Human
- Omim: 601246 Human
- SwissProt: Q13111 Human
- Unigene: 79018 Human
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Alternative names
- CAF 1 antibody
- CAF 1 subunit A antibody
- CAF antibody
see all
Images
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Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)All lanes : Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate, treated with hydroxyourea
Lane 3 : HeLa cell line lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 107 kDa -
![Flow Cytometry - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)](https://www.abcam.com/ps/products/126/ab126625/Images/ab126625-215918-anti-p150-caf1-antibody-epr55762-flow-cytometry.jpg)
Flow Cytometry - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)Overlay histogram showing HeLa cells stained with ab126625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126625, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)](https://www.abcam.com/ps/products/126/ab126625/Images/ab126625-140623-anti-p150-caf1-antibody-epr55762-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)Formalin-fixed, paraffin-embedded human colon tissue stained for p150 CAF1/CAF with ab126625 (1/100 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)](https://www.abcam.com/ps/products/126/ab126625/Images/ab126625-140622-anti-p150-caf1-antibody-epr55762-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)Formalin-fixed, paraffin-embedded human tonsil tissue stained for p150 CAF1/CAF with ab126625 (1/100 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)](https://www.abcam.com/ps/products/126/ab126625/Images/ab126625-5-benefits-of-recombinant-antibodies.png)
Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab126625 has been referenced in 9 publications.
- Huang J et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
- Morra F et al. CAF-1 Subunits Levels Suggest Combined Treatments with PARP-Inhibitors and Ionizing Radiation in Advanced HNSCC. Cancers (Basel) 11:N/A (2019). PubMed: 31627329
- Zheng L et al. CHAF1A interacts with TCF4 to promote gastric carcinogenesis via upregulation of c-MYC and CCND1 expression. EBioMedicine 38:69-78 (2018). PubMed: 30449701
- Roy S et al. SIRF: Quantitative in situ analysis of protein interactions at DNA replication forks. J Cell Biol 217:1521-1536 (2018). PubMed: 29475976
- Yang C et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res 45:739-748 (2017). PubMed: 27794043
- Xu M et al. Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma. Onco Targets Ther 9:4023-35 (2016). WB, IHC-P ; Human . PubMed: 27445493
- Wang J et al. A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two-cell embryos. EMBO Rep 17:1452-1470 (2016). PubMed: 27496889
- Wang L et al. Mitogen-activated protein kinase kinase 3 induces cell cycle arrest via p38 activation mediated Bmi-1 downregulation in hepatocellular carcinoma. Mol Med Rep 13:243-8 (2016). PubMed: 26573508
- Barbieri E et al. Histone chaperone CHAF1A inhibits differentiation and promotes aggressive neuroblastoma. Cancer Res 74:765-74 (2014). PubMed: 24335960