Anti-P15RS antibody (ab88304)
Key features and details
- Mouse polyclonal to P15RS
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P15RS antibody -
Description
Mouse polyclonal to P15RS -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human P15RS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: human liver tissue lysate, P15RS transfected 293T cell lysate ICC/IF: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
ICC/IF |
Notes |
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WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa for isoform 1 and 33 kDa for isoform 2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle. -
Sequence similarities
Belongs to the UPF0400 (RTT103) family.
Contains 1 CID domain. - Information by UniProt
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Database links
- Entrez Gene: 55197 Human
- Omim: 610347 Human
- SwissProt: Q96P16 Human
- Unigene: 464912 Human
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Alternative names
- C77387 antibody
- CDKN2B-related protein antibody
- Cyclin dependent kinase 2B inhibitor related protein antibody
see all
Images
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Anti-P15RS antibody (ab88304) at 1 µg/ml + human liver tissue lysate at 50 µg
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
All lanes : Anti-P15RS antibody (ab88304) at 1 µg/ml
Lane 1 : P15RS transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 33 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
ab88304, at 10 µg/ml, staining P15RS in HeLa cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab88304 has not yet been referenced specifically in any publications.