Key features and details
- Mouse polyclonal to P15RS
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P15RS antibody
DescriptionMouse polyclonal to P15RS
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length human P15RS protein (AAH10136.1; Q96P16-2).
- WB: human liver tissue lysate, P15RS transfected 293T cell lysate ICC/IF: HeLa cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa for isoform 1 and 33 kDa for isoform 2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInteracts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle.
Sequence similaritiesBelongs to the UPF0400 (RTT103) family.
Contains 1 CID domain.
- Information by UniProt
- C77387 antibody
- CDKN2B-related protein antibody
- Cyclin dependent kinase 2B inhibitor related protein antibody
Anti-P15RS antibody (ab88304) at 1 µg/ml + human liver tissue lysate at 50 µg
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
All lanes : Anti-P15RS antibody (ab88304) at 1 µg/ml
Lane 1 : P15RS transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 33 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ab88304, at 10 µg/ml, staining P15RS in HeLa cell by Immunofluorescence.
ab88304 has not yet been referenced specifically in any publications.