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Hi, I appreciate your prompt response. The samples are human cell extracts. I have not attempted other concentrations since the product sheet suggested the dilution of 1:20,000. Normally, I try to "optimize" the antibody concentration if there is no recommendations. Since this antibody had been tested and verified, I only tested the one concentration (1:10,000). I informed my supervisor of the situation and mentioned that Abcam is willing to send another lot. Please let me know if the situation is true. Thanks for all your help!
Asked on Jan 13 2012
Thank you for contacting us. That is correct. I will process that for you as soon as you are able to send me the ooriginal order number or PO used to purchase this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jan 13 2012