Product nameAnti-p18 INK4c/CDKN2C antibody
See all p18 INK4c/CDKN2C primary antibodies
DescriptionRabbit polyclonal to p18 INK4c/CDKN2C
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Rat, Human
- Recombinant Human p18 INK4c/CDKN2C protein (ab119460) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Rat Thymus Tissue Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM) HeLa Whole Cell
This product was previously labelled as p18 INK4c
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab66748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [18P118 (same as DCS-118)] to p18 INK4c/CDKN2C (ab3216) as Capture.
FunctionInteracts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Tissue specificityHighest levels found in skeletal muscle. Also found in pancreas and heart.
Sequence similaritiesBelongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats.
- Information by UniProt
- CDK6 inhibitor p18 antibody
- CDKN 2C antibody
- CDKN 6 antibody
Standard Curve for p18 INK4c/CDKN2C (Analyte: p18 INK4c/CDKN2C protein (T7 Tag) (ab40127)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [18P118 (same as DCS-118)] to p18 INK4c/CDKN2C (ab3216) at 5ug/ml and Detector Antibody Rabbit polyclonal to p18 INK4c/CDKN2C (ab66748) at 0.5ug/ml.
All lanes : Anti-p18 INK4c/CDKN2C antibody (ab66748) at 1 µg/ml
Lane 1 : Rat Thymus Tissue Lysate
Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab66748 has not yet been referenced specifically in any publications.