Recombinant
RabMAb

Recombinant Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)

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Overview

  • Product name

    Anti-p18 INK4c/CDKN2C antibody [EPR15891]
    See all p18 INK4c/CDKN2C primary antibodies

  • Description

    Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human p18 INK4c/CDKN2C aa 1-150. The exact sequence is proprietary.
    Database link: P42773

  • Positive control

    • 293, Ramos, HeLa, Rat kidney, Rat spleen and NIH 3T3 lysates; Human brain and glioma tissues; HeLa cells.

  • General notes

     

     

     This product was previously labelled as p18 INK4c

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP 1/30.
ICC/IF 1/100.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.

  • Tissue specificity

    Highest levels found in skeletal muscle. Also found in pancreas and heart.

  • Sequence similarities

    Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CDK6 inhibitor p18 antibody
    • CDKN 2C antibody
    • CDKN 6 antibody
    • Cdkn2c antibody
    • CDKN6 antibody
    • CDN2C_HUMAN antibody
    • Cyclin dependent inhibitor antibody
    • Cyclin dependent kinase 4 inhibitor C antibody
    • Cyclin dependent kinase 6 inhibitor antibody
    • Cyclin dependent kinase 6 inhibitor p18 antibody
    • Cyclin dependent kinase inhibitor 2C (p18 inhibits CDK4) antibody
    • Cyclin dependent kinase inhibitor 2C antibody
    • Cyclin-dependent kinase 4 inhibitor C antibody
    • Cyclin-dependent kinase 6 inhibitor antibody
    • INK4C antibody
    • OTTHUMP00000046546 antibody
    • p18 antibody
    • p18 inhibits CDK4 antibody
    • p18 INK4c antibody
    • p18 INK6 antibody
    • p18-INK4c antibody
    • p18-INK6 antibody
    see all

Images

  • Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : NIH 3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling p18 INK4C/CDKN2C with ab192239 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    Negative controls: anti-p18 INK4C/CDKN2C at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

     

  • Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)

    Western blot analysis of immunoprecipitation pellet from HeLa lysate immunoprecipitated using ab192239 at 1/30 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)

    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
    All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 18 kDa

References

This product has been referenced in:

  • Prikrylova T  et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). Read more (PubMed: 31363131) »
  • Brock M  et al. Analysis of hypoxia-induced noncoding RNAs reveals metastasis-associated lung adenocarcinoma transcript 1 as an important regulator of vascular smooth muscle cell proliferation. Exp Biol Med (Maywood) 242:487-496 (2017). Read more (PubMed: 28056547) »
See all 4 Publications for this product

Publishing research using ab192239? Please let us know so that we can cite the reference in this datasheet.

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