Recombinant Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-389690-cdkn2c-257887-192239-full--anti-p18-ink4ccdkn2c-antibody-epr15891-western-blot-human-lysate.jpg "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C
- Suitable for: WB, IP, ICC/IF, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-p18 INK4c/CDKN2C antibody [EPR15891]
See all p18 INK4c/CDKN2C primary antibodies -
Description
Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEL-293T, Ramos, HeLa, Jurkat, Rat kidney, Rat spleen and NIH 3T3 lysates. IHC-P: Human brain and glioma tissues; ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
EPR15891 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
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| IP |
1/30.
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| ICC/IF |
1/100.
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| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
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IP
1/30. |
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ICC/IF
1/100. |
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. -
Tissue specificity
Highest levels found in skeletal muscle. Also found in pancreas and heart. -
Sequence similarities
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats. - Information by UniProt
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Database links
- Entrez Gene: 1031 Human
- Entrez Gene: 12580 Mouse
- Entrez Gene: 54238 Rat
- Omim: 603369 Human
- SwissProt: P42773 Human
- SwissProt: Q60772 Mouse
- Unigene: 525324 Human
- Unigene: 728783 Human
see all -
Alternative names
- CDK6 inhibitor p18 antibody
- CDKN 2C antibody
- CDKN 6 antibody
see all
Images
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Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CDKN2C knockout HeLa cell lysate
Lane 3 : Rat Kidney cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDaLanes 1-4: Merged signal (red and green). Green - ab192239 observed at 18 kDa. Red - loading control ab8245 observed at 37 kDa.
ab192239 Anti-p18 INK4c/CDKN2C antibody [EPR15891] was shown to specifically react with Cyclin Dependent Kinase Inhibitor 2C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265031 (knockout cell lysate ab257887) was used. Wild-type and Cyclin Dependent Kinase Inhibitor 2C knockout samples were subjected to SDS-PAGE. ab192239 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-253283-anti-p18-ink4c-antibody-epr15891-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling p18 INK4C/CDKN2C with ab192239 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-p18 INK4C/CDKN2C at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. -
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230025-anti-p18-ink4c-antibody-epr15891-western-blot.jpg)
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Rat spleen lysate
Lane 3 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 18 kDa -
![Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230029-anti-p18-ink4c-antibody-epr15891-immunoprecipitation.jpg)
Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Western blot analysis of immunoprecipitation pellet from HeLa lysate immunoprecipitated using ab192239 at 1/30 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230027-anti-p18-ink4c-antibody-epr15891-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230026-anti-p18-ink4c-antibody-epr15891-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-230024-anti-p18-ink4c-antibody-epr15891-western-blot.jpg)
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 18 kDa -
![Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)](https://www.abcam.com/ps/products/192/ab192239/Images/ab192239-9-benefits-of-recombinant-antibodies.png)
Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (10)
ab192239 has been referenced in 10 publications.
- Li Y et al. Stabilization of p18 by deubiquitylase CYLD is pivotal for cell cycle progression and viral replication. NPJ Precis Oncol 5:14 (2021). PubMed: 33654169
- Lefèvre L et al. Kidney inflammaging is promoted by CCR2+?macrophages and tissue-derived micro-environmental factors. Cell Mol Life Sci 78:3485-3501 (2021). PubMed: 33313981
- Wu H et al. miR-34a in extracellular vesicles from bone marrow mesenchymal stem cells reduces rheumatoid arthritis inflammation via the cyclin I/ATM/ATR/p53 axis. J Cell Mol Med 25:1896-1910 (2021). PubMed: 33465281
- Liu M et al. PRMT5-dependent transcriptional repression of c-Myc target genes promotes gastric cancer progression. Theranostics 10:4437-4452 (2020). PubMed: 32292506
- Eller C et al. A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor. Nat Commun 11:2707 (2020). PubMed: 32483149
- Meadows V et al. Downregulation of hepatic stem cell factor by Vivo-Morpholino treatment inhibits mast cell migration and decreases biliary damage/senescence and liver fibrosis in Mdr2-/- mice. Biochim Biophys Acta Mol Basis Dis 1865:165557 (2019). PubMed: 31521820
- Prikrylova T et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). PubMed: 31363131
- Brock M et al. Analysis of hypoxia-induced noncoding RNAs reveals metastasis-associated lung adenocarcinoma transcript 1 as an important regulator of vascular smooth muscle cell proliferation. Exp Biol Med (Maywood) 242:487-496 (2017). PubMed: 28056547
- Zhang S et al. lncRNA Up-Regulated in Nonmuscle Invasive Bladder Cancer Facilitates Tumor Growth and Acts as a Negative Prognostic Factor of Recurrence. J Urol 196:1270-8 (2016). PubMed: 27267320
- Wang L et al. Mitogen-activated protein kinase kinase 3 induces cell cycle arrest via p38 activation mediated Bmi-1 downregulation in hepatocellular carcinoma. Mol Med Rep 13:243-8 (2016). PubMed: 26573508
