Recombinant Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-389779-cdkn2c-257887-192239-full--anti-p18-ink4ccdkn2c-antibody-epr15891-western-blot-human-lysate.jpg "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free
See all p18 INK4c/CDKN2C primary antibodies -
Description
Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human glioma tissue; Mouse brain tissue. WB: HeLa, rat kidney and Jurkat cell lysates. ICC/IF: HeLa cells. IP: HeLa cell lysate.
-
General notes
ab232631 is the carrier-free version of ab192239.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
EPR15891 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
Target
-
Function
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. -
Tissue specificity
Highest levels found in skeletal muscle. Also found in pancreas and heart. -
Sequence similarities
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats. - Information by UniProt
-
Database links
- Entrez Gene: 1031 Human
- Entrez Gene: 12580 Mouse
- Entrez Gene: 54238 Rat
- Omim: 603369 Human
- SwissProt: P42773 Human
- SwissProt: Q60772 Mouse
- Unigene: 525324 Human
- Unigene: 728783 Human
see all -
Alternative names
- CDK6 inhibitor p18 antibody
- CDKN 2C antibody
- CDKN 6 antibody
see all
Images
-
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)All lanes : Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CDKN2C knockout HeLa cell lysate
Lane 3 : Rat Kidney cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab192239).
Lanes 1-4: Merged signal (red and green). Green - ab192239 observed at 18 kDa. Red - loading control ab8245 observed at 37 kDa.
ab192239 Anti-p18 INK4c/CDKN2C antibody [EPR15891] was shown to specifically react with Cyclin Dependent Kinase Inhibitor 2C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265031 (knockout cell lysate ab257887) was used. Wild-type and Cyclin Dependent Kinase Inhibitor 2C knockout samples were subjected to SDS-PAGE. ab192239 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-4-ab192239253283192239.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling p18 INK4C/CDKN2C with ab192239 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-p18 INK4C/CDKN2C at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
-
![Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-3-ab192239230029ab192239f.jpg)
Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Western blot analysis of immunoprecipitation pellet from HeLa lysate immunoprecipitated using ab192239 at 1/30 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-2-ab192239230026ab192239c.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-308767-239.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-6-benefits-of-recombinant-antibodies.png)
Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab232631 has not yet been referenced specifically in any publications.
