Recombinant Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)
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Overview
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Product name
Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free
See all p18 INK4c/CDKN2C primary antibodies -
Description
Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human p18 INK4c/CDKN2C aa 1-150. The exact sequence is proprietary.
Database link: P42773 -
Positive control
- IHC-P: Human glioma tissue.
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General notes
ab232631 is the carrier-free version of ab192239 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232631 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as p18 INK4c
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15891 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab232631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. -
Tissue specificity
Highest levels found in skeletal muscle. Also found in pancreas and heart. -
Sequence similarities
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats. - Information by UniProt
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Database links
- Entrez Gene: 1031 Human
- Entrez Gene: 12580 Mouse
- Entrez Gene: 54238 Rat
- Omim: 603369 Human
- SwissProt: P42773 Human
- SwissProt: Q60772 Mouse
- Unigene: 525324 Human
- Unigene: 728783 Human
see all -
Alternative names
- CDK6 inhibitor p18 antibody
- CDKN 2C antibody
- CDKN 6 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-4-ab192239253283192239.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling p18 INK4C/CDKN2C with ab192239 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-p18 INK4C/CDKN2C at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
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![Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-3-ab192239230029ab192239f.jpg)
Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Western blot analysis of immunoprecipitation pellet from HeLa lysate immunoprecipitated using ab192239 at 1/30 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-2-ab192239230026ab192239c.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)](https://www.abcam.com/ps/products/232/ab232631/Images/ab232631-308767-239.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
References
ab232631 has not yet been referenced specifically in any publications.
