Recombinant
RabMAb

Recombinant Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free (ab232631)

Overview

  • Product name

    Anti-p18 INK4c/CDKN2C antibody [EPR15891] - BSA and Azide free
    See all p18 INK4c/CDKN2C primary antibodies
  • Description

    Rabbit monoclonal [EPR15891] to p18 INK4c/CDKN2C - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human p18 INK4c/CDKN2C aa 1-150. The exact sequence is proprietary.
    Database link: P42773

  • Positive control

    • IHC-P: Human glioma tissue.
  • General notes

    ab232631 is the carrier-free version of ab192239 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232631 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as p18 INK4c

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
  • Tissue specificity

    Highest levels found in skeletal muscle. Also found in pancreas and heart.
  • Sequence similarities

    Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDK6 inhibitor p18 antibody
    • CDKN 2C antibody
    • CDKN 6 antibody
    • Cdkn2c antibody
    • CDKN6 antibody
    • CDN2C_HUMAN antibody
    • Cyclin dependent inhibitor antibody
    • Cyclin dependent kinase 4 inhibitor C antibody
    • Cyclin dependent kinase 6 inhibitor antibody
    • Cyclin dependent kinase 6 inhibitor p18 antibody
    • Cyclin dependent kinase inhibitor 2C (p18 inhibits CDK4) antibody
    • Cyclin dependent kinase inhibitor 2C antibody
    • Cyclin-dependent kinase 4 inhibitor C antibody
    • Cyclin-dependent kinase 6 inhibitor antibody
    • INK4C antibody
    • OTTHUMP00000046546 antibody
    • p18 antibody
    • p18 inhibits CDK4 antibody
    • p18 INK4c antibody
    • p18 INK6 antibody
    • p18-INK4c antibody
    • p18-INK6 antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling p18 INK4C/CDKN2C with ab192239 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    Negative controls: anti-p18 INK4C/CDKN2C at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).

  • Western blot analysis of immunoprecipitation pellet from HeLa lysate immunoprecipitated using ab192239 at 1/30 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192239).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab232631 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232631.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up