• Product name
  • Description
    Rabbit polyclonal to p19 INK4d
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 22-51 of Human p19 INK4d (UniProt ID: P55273) conjugated to KLH.

  • Positive control
    • Human testis lysate; testis tissue IF: HeLa cell line



Our Abpromise guarantee covers the use of ab102842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance
    Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • CDK inhibitor p19INK4d antibody
    • CDKN2D antibody
    • cell cycle inhibitor, Nur77 associating protein antibody
    • Cyclin dependent kinase inhibitor 2D antibody
    • Cyclin-dependent kinase 4 inhibitor D antibody
    • Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) antibody
    • inhibitor of cyclin-dependent kinase 4d antibody
    • INK4D antibody
    • MGC109660 antibody
    • MGC137547 antibody
    • MGC73010 antibody
    • p19 antibody
    • p19-INK4D antibody
    • p19INK4d antibody
    • Similar to cyclin-dependent kinase inhibitor 2D antibody
    see all


  • Anti-p19 INK4d antibody (ab102842) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

    Predicted band size: 18 kDa

  • ab102842, at a 1/50 dilution, staining P19 INK4d in formalin fixed, paraffin embedded testis tissue by Immunohistochemistry.
  • ICC/IF image of ab102842 stained HeLa cells. The cells were 4% paraformaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab102842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Rabachini T  et al. BOK promotes chemical-induced hepatocarcinogenesis in mice. Cell Death Differ 25:706-718 (2018). WB, IHC-P ; Mouse . Read more (PubMed: 29229991) »
  • Tanaka T  et al. The efficacy of the cyclin-dependent kinase 4/6 inhibitor in endometrial cancer. PLoS One 12:e0177019 (2017). WB ; Human . Read more (PubMed: 28472136) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I have processed your request to receive a new vial of ab102842 to try further dilutions of the antibody. Your new order number is * and you should receive that antibody tomorrow.

If there is anything else I ...

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Thank you for your reply.

The recommended dilutions on the datasheets are only intended as a useful starting point for our customers, we strongly advise to optimize the antibody conditions for use in your own experiments.

I would be...

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Thank you for your reply.

Based on the information that you have provided, I think that the following protocol suggestions may prove to be successful:

1 - Switch from a 5% milk to a 5% BSA block, this should help to cut down on back...

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Thank you for your reply.

I would be very happy to try and help resolve this issue, if we cannot reduce the levels of background you are getting using ab102842, then I will be happy to send a replacement. To be able to help further in this ma...

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Thank you for contacting us.

Yes, the antibody would still work if residues 47-51 are missing from the immunogen region.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice o...

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Thank you for your response and for passing some useful details to me. RT-PCR data should be treated carefully since, there is no clear correlation between mRNA and protein levels and sometimes it is not easy to predict the protein expression level...

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