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    p19-ink4d-antibody-ab262871.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Ink4
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Anti-p19 INK4d antibody (ab262871)

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Immunocytochemistry/ Immunofluorescence - Anti-p19 INK4d antibody (ab262871)

    Key features and details

    • Rabbit polyclonal to p19 INK4d
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    Protein
    Product image
    Recombinant Human p19 INK4d protein (ab85658)

    View more associated products

    Overview

    • Product name

      Anti-p19 INK4d antibody
    • Description

      Rabbit polyclonal to p19 INK4d
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment corresponding to Human p19 INK4d aa 50-150.
      Database link: P55273

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • ICC/IF: A431 cells.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Ink4
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Cycle Inhibitors
      • Ink4
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • INK4a/Arf

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Recombinant Protein

      • Recombinant Human p19 INK4d protein (ab85658)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab262871 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use a concentration of 1 - 4 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100

    Notes
    ICC/IF
    Use a concentration of 1 - 4 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100

    Target

    • Relevance

      Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
    • Cellular localization

      Cytoplasmic and Nuclear
    • Database links

      • Entrez Gene: 1032 Human
      • Omim: 600927 Human
      • SwissProt: P55273 Human
      • Alternative names

        • CDK inhibitor p19INK4d antibody
        • CDKN2D antibody
        • cell cycle inhibitor, Nur77 associating protein antibody
        • Cyclin dependent kinase inhibitor 2D antibody
        • Cyclin-dependent kinase 4 inhibitor D antibody
        • Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) antibody
        • inhibitor of cyclin-dependent kinase 4d antibody
        • INK4D antibody
        • MGC109660 antibody
        • MGC137547 antibody
        • MGC73010 antibody
        • p19 antibody
        • p19-INK4D antibody
        • p19INK4d antibody
        • Similar to cyclin-dependent kinase inhibitor 2D antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-p19 INK4d antibody (ab262871)
        Immunocytochemistry/ Immunofluorescence - Anti-p19 INK4d antibody (ab262871)
        PFA-fixed, Triton X-100 permeabilized human A-431 cells stained for p19 INK4d (green) using ab262871 at 4 µg/ml in ICC/IF.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab262871? Please let us know so that we can cite the reference in this datasheet.

      ab262871 has not yet been referenced specifically in any publications.

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