Product namep19 INK4d overexpression 293T lysate (whole cell)
See all p19 INK4d lysates
Descriptionp19 INK4d overexpression lysate
ab94315 is a 293T cell transfected lysate in which Human p19 INK4d has been transiently over-expressed using a pCMV-p19 INK4d plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundCyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
Our Abpromise guarantee covers the use of ab94315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94315 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-p19 INK4d antibody (ab77223) at 1/500 dilution
Lane 1 :
p19 INK4d overexpression 293T lysate (whole cell) (ab94315)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94315 has not yet been referenced specifically in any publications.