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  1. Link

    p21-antibody-epr362-ab109520.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Cip / Kip
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-p21 antibody [EPR362] (ab109520)

  • Datasheet
  • SDS
Reviews (14) Submit a question References (257)

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Western blot - Anti-p21 antibody [EPR362] (ab109520)
  • Western blot - Anti-p21 antibody [EPR362] (ab109520)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)
  • Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody [EPR362] (ab109520)
  • Immunoprecipitation - Anti-p21 antibody [EPR362] (ab109520)
  • Flow Cytometry (Intracellular) - Anti-p21 antibody [EPR362] (ab109520)
  • Western blot - Anti-p21 antibody [EPR362] (ab109520)
  • Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody [EPR362] (ab109520)
  • Western blot - Anti-p21 antibody [EPR362] (ab109520)
  • Western blot - Anti-p21 antibody [EPR362] (ab109520)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)
  • Western blot - Anti-p21 antibody [EPR362] (ab109520)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)
  • Western blot - Anti-p21 antibody [EPR362] (ab109520)
  • Anti-p21 antibody [EPR362] (ab109520)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR362] to p21
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

You may also be interested in

Primary
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Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal (ab108349)
Primary
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Anti-p21 antibody [EPR362] - BSA and Azide free (ab218311)
Knockout
Product image
Human CDKN1A knockout HeLa cell line (ab255349)

View more associated products

Overview

  • Product name

    Anti-p21 antibody [EPR362]
    See all p21 primary antibodies
  • Description

    Rabbit monoclonal [EPR362] to p21
  • Host species

    Rabbit
  • Specificity

    Expression levels of the target protein vary between different tissue/cell lines and in some cases induction may be required before a signal is observed.
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, HeLa, HEK293, HUVEC, LnCaP, U87 MG or HEK-293T cell lysates. IHC-P: Human cervical carcinoma or papillary carcinoma of thyroid gland tissues. ICC/IF: MCF-7 cells. Flow Cyt (intra): HeLa cells. IP: HEK-293 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR362
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Cip / Kip
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Cip/Kip
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Cip/kip
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cancer proteins ELISA kits
    • Signal Transduction
    • Antibodies
    • p21

Associated products

  • Alternative Versions

    • Anti-p21 antibody [EPR362] - BSA and Azide free (ab218311)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • HEK-293 whole cell lysate (ab7902)
  • Recombinant Protein

    • Recombinant Human p21 protein (ab56278)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100.
ICC/IF (3)
1/1000.

For unpurified use at 1/50 - 1/100.

WB (5)
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IP
1/10 - 1/100.
IHC-P (3)
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
Flow Cyt (Intra)
1/100.
ICC/IF
1/1000.

For unpurified use at 1/50 - 1/100.

WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
  • Tissue specificity

    Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.
  • Sequence similarities

    Belongs to the CDI family.
  • Domain

    The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
  • Post-translational
    modifications

    Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
    Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P38936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1026 Human
    • Omim: 116899 Human
    • SwissProt: P38936 Human
    • Unigene: 370771 Human
    • Alternative names

      • CAP20 antibody
      • CDK-interacting protein 1 antibody
      • CDKI antibody
      • CDKN1 antibody
      • Cdkn1a antibody
      • CDN1A_HUMAN antibody
      • CIP1 antibody
      • Cyclin Dependent Kinase Inhibitor 1A antibody
      • Cyclin-dependent kinase inhibitor 1 antibody
      • Cyclin-dependent kinase inhibitor 1A (P21) antibody
      • Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody
      • DNA Synthesis Inhibitor antibody
      • MDA-6 antibody
      • MDA6 antibody
      • Melanoma differentiation-associated protein 6 antibody
      • Melanoma differentiation-associated protein antibody
      • p21 antibody
      • P21 protein antibody
      • p21CIP1 antibody
      • p21Cip1/Waf1 antibody
      • p21WAF antibody
      • PIC1 antibody
      • SDI1 antibody
      • SLC12A9 antibody
      • WAF1 antibody
      • Wild type p53 activated fragment 1 (WAF1) antibody
      • Wild type p53 activated fragment 1 antibody
      • Wildtype p53-activated fragment 1 antibody
      see all

    Images

    • Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Western blot - Anti-p21 antibody [EPR362] (ab109520)
      All lanes : Anti-p21 antibody [EPR362] (ab109520) at 1/1000 dilution

      Lane 1 : wild-type HeLa Vehicle Control Fluvastatin (0 uM, 24 h) cell lysate
      Lane 2 : wild-type HeLa Treated Fluvastatin (50 uM, 24 h) cell lysate
      Lane 3 : CDKN1A knockout HeLa Vehicle Control Fluvastatin (0 uM, 24 h) cell lysate
      Lane 4 : CDKN1A knockout HeLa Treated Fluvastatin (50 uM, 24 h) cell lysate
      Lane 5 : MCF7 cell lysate
      Lane 6 : SH-SY5Y cell lysate

      Performed under reducing conditions.

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      False colour image of Western blot: Anti-p21 antibody [EPR362] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109520 was shown to bind specifically to p21. A band was observed at 21 kDa in wild-type HeLa cell lysates with no signal observed at this size in CDKN1A knockout cell line ab255349 (knockout cell lysate ab263812). To generate this image, wild-type and CDKN1A knockout cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Western blot - Anti-p21 antibody [EPR362] (ab109520)

      Lane 1: Wild-type DLD-1 cell lysate (20 µg)

      Lane 2: Wild-type DLD-1 20 μM 2,3-DCPE for 16hrs treated cell lysate (20 µg)

      Lane 3: p21 knockout DLD-1 cell lysate (20 µg)

      Lane 4: p21 knockout 20 μM 2,3-DCPE for 16hrs DLD-1 cell lysate (20 µg)

      Lane 5: HT1080 cell lysate (20 μg)


      Lanes 1 - 5: Merged signal (red and green). Green - ab109520 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109520 was shown to specifically recognize p21 in wild-type DLD-1 cells treated with 20 μM 2,3-DCPE. No band was observed when p21 knockout samples +/- 2,3-DCPE treatment were used. Wild-type and p21 knockout samples were subjected to SDS-PAGE. ab109520 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling p21 with purified ab109520 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody [EPR362] (ab109520)
      Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody [EPR362] (ab109520)
      Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling p21 with ab109520 at 1/500 (2 μg/ml). ab150077, Alexa Fluor®488 Goat anti-Rabbit at 1/1000 (2 μg/ml) was used as secondary antibody. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml) was used as counterstain AbID. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Nuclei were counter stained blue with DAPI.

      Confocal image showing nuclear staining on MCF7 cell line. 

    • Immunoprecipitation - Anti-p21 antibody [EPR362] (ab109520)
      Immunoprecipitation - Anti-p21 antibody [EPR362] (ab109520)

      ab109520 (purified) at 1/50 immunoprecipitating p21 in HEK293 whole cell lysate.

      Lane 1 (input): HEK293 whole cell lysate (10µg)

      Lane 2 (+): ab109520 + HEK293 whole cell lysate (10µg).

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109520 in HEK293 whole cell lysate.

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Flow Cytometry (Intracellular) - Anti-p21 antibody [EPR362] (ab109520)
      Flow Cytometry (Intracellular) - Anti-p21 antibody [EPR362] (ab109520)

      Overlay histogram showing HeLa cells stained with unpurified ab109520 (red line). The cells were fixed with 80% methanol (5 min) then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab109520, 1/100) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. 

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Different batches of ab109520 were tested on MCF7 (Human breast adenocarcinoma epithelial cell) lysate at 0.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 21 kDa.
    • Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody [EPR362] (ab109520)
      Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody [EPR362] (ab109520)

      Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labeling p21 with purified ab109520 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

      Control 1: primary antibody (1/1000) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Western blot - Anti-p21 antibody [EPR362] (ab109520)

      Lane 1: Wild-type DLD-1 cell lysate (20 µg)
      Lane 2: Wild-type DLD-1 20 μM 2,3-DCPE for 16hrs treated cell lysate (20 µg)
      Lane 3: p21 knockout DLD-1 cell lysate (20 µg)
      Lane 4: p21 knockout 20 μM 2,3-DCPE for 16hrs DLD-1 cell lysate (20 µg)
      Lane 5: HT1080 cell lysate (20 μg)

      Lanes 1 - 5: Merged signal (red and green). Green - ab109520 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

      This western blot image is a comparison between ab109520 and a competitor's top cited rabbit polyclonal antibody.

    • Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Western blot - Anti-p21 antibody [EPR362] (ab109520)
      All lanes : Anti-p21 antibody [EPR362] (ab109520) at 1/2000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : HEK293 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human papillary carcinoma of the thyroid gland tissue labelling p21 with unpurified ab109520 at a dilution of 1/100.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Anti-p21 antibody [EPR362] (ab109520) at 1/10000 dilution (purified) + LnCaP cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR362] (ab109520)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling p21 with unpurified ab109520 at a dilution of 1/100.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-p21 antibody [EPR362] (ab109520)
      Western blot - Anti-p21 antibody [EPR362] (ab109520)
      All lanes : Anti-p21 antibody [EPR362] (ab109520) at 1/1000 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : LnCap cell lysate
      Lane 5 : U87 MG cell lysate
      Lane 6 : 293T cell lsyate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

      Predicted band size: 21 kDa

    • Anti-p21 antibody [EPR362] (ab109520)
      Anti-p21 antibody [EPR362] (ab109520)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (257)

    Publishing research using ab109520? Please let us know so that we can cite the reference in this datasheet.

    ab109520 has been referenced in 257 publications.

    • Zhang Y  et al. Long Non-coding RNA CASC15 Promotes Intrahepatic Cholangiocarcinoma Possibly through Inducing PRDX2/PI3K/AKT Axis. Cancer Res Treat 53:184-198 (2021). PubMed: 33017884
    • Niu L  et al. Sericin inhibits MDA-MB-468 cell proliferation via the PI3K/Akt pathway in triple-negative breast cancer. Mol Med Rep 23:N/A (2021). PubMed: 33313947
    • Yang Y & Min Z Effect of long non-coding RNA AK021443 on promoting hepatic fibrosis in vitro. Mol Med Rep 23:N/A (2021). PubMed: 33495828
    • Ji H  et al. SP1 induced long non-coding RNA AGAP2-AS1 promotes cholangiocarcinoma proliferation via silencing of CDKN1A. Mol Med 27:10 (2021). PubMed: 33522895
    • Wang H  et al. Knockdown of Dual Oxidase 1 (DUOX1) Promotes Wound Healing by Regulating Reactive Oxygen Species (ROS) by Activation of Nuclear Kactor kappa B (NF-?B) Signaling. Med Sci Monit 27:e926492 (2021). PubMed: 33563887
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 14 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p21 antibody [EPR362]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6
    Permeabilization
    No
    Specification
    skin
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Barbara Schädl

    Verified customer

    Submitted Jun 17 2022

    Western blot abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SK-Mel 28 (Melanoma cell line))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    SK-Mel 28 (Melanoma cell line)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 13 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cervical cancer cell)
    Permeabilization
    Yes - Triton-X
    Specification
    Cervical cancer cell
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Mar 18 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Cervical cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH8, EDTA
    Permeabilization
    No
    Specification
    Cervical cancer
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Mar 18 2021

    Western blot abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (SHSY5Y cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycin gel)
    Loading amount
    40 µg
    Specification
    SHSY5Y cells
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Feb 03 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Cervix)
    Permeabilization
    No
    Specification
    Cervix
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Submitted Dec 08 2020

    Immunocytochemistry abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HaCaT (Keratinocytes))
    Permeabilization
    No
    Specification
    HaCaT (Keratinocytes)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 20°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 23 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Melanoma cells)
    Permeabilization
    Yes - Triton X-100
    Specification
    Melanoma cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 26 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Melanoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Specification
    Melanoma
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 22 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-p21 antibody [EPR362]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin nevus)
    Specification
    Skin nevus
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 18 2020

    1-10 of 14 Abreviews or Q&A

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