The antibody detects p21 after short exposure times. We used HeLa cell cycle lysates from Abcam (ab136811) to check for cell cycle dependent p21 expression. Additionally we induced DNA damage response in HCT116 wt and MEF cells by CPT-11 treatment and used HCT116 p21-/- cells a negative control.
Unfortunately the antibody gives some unspecific signals at 20 kDa in HeLa cells blocked in G2 phase.Thus it's hard to distinguish between specific and unspecific signal in these cells.
The antibody work well for the HCT116 cells. But does not work in murine cells. We used 15 ´g HeLa and MEF cell lysates, but only 7 ´g of HCT cell lysates.
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
Mr. Christian Marx
Submitted Jan 15 2014
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