Western blot abreview for Anti-p21 antibody [EPR362]

Good
Abreviews
Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (MCF-7)
Specification
MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL select
Bands
Specific: 15-20 kDa Non-specific: 60 kDa
Exposure
3 minute(s) and 0 second(s)
Positive control
MCF-7

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Submitted Aug 04 2014

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