Western blot abreview for Anti-p21 antibody [EPR362]

Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
MEF
Blocking step
ODYSSEY BLOCKING BUFFER as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Donkey ANTI RABIT IRDye 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 680

Detection

Detection method
Li-Cor
Exposure
25 minute(s) and 0 second(s)
Positive control
tubulin

Additional data

Additional Notes
The membrane was blotted agains anti tubulin (ab6046) and anti p21 (ab109520).
Abcam response
Thank you so much for testing this antibody in mouse. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species. The customer received a discount on a future antibody purchase for testing this product. We welcome additional feedback from other customers as well.

Muhammed Zoabi

Verified customer

Submitted Apr 27 2017

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