Anti-p21 antibody [HUGO291] (ab107099)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)](https://www.abcam.com/ps/products/107/ab107099/Images/ab107099-266544-anti-p21-antibody-hugo291-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)")
Key features and details
- Rat monoclonal [HUGO291] to p21
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-p21 antibody [HUGO291]
See all p21 primary antibodies -
Description
Rat monoclonal [HUGO291] to p21 -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to p21. HIS-GST-mp21
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Positive control
- Mouse skin papilloma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
HUGO291 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab107099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50. Predicted molecular weight: 18 kDa.
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| IHC-P | (1) |
1/100.
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| Notes |
|---|
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WB
1/50. Predicted molecular weight: 18 kDa. |
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IHC-P
1/100. |
Target
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Function
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. -
Tissue specificity
Expressed in all adult human tissues, with 5-fold lower levels observed in the brain. -
Sequence similarities
Belongs to the CDI family. -
Domain
The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex. -
Post-translational
modificationsPhosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1026 Human
- Entrez Gene: 12575 Mouse
- Omim: 116899 Human
- SwissProt: P38936 Human
- SwissProt: P39689 Mouse
- Unigene: 370771 Human
- Unigene: 195663 Mouse
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Alternative names
- CAP20 antibody
- CDK-interacting protein 1 antibody
- CDKI antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse p21WT fibrosarcoma and mouse p21KO fibrosarcoma tissue sections labeling p21 with ab107099.
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![Western blot - Anti-p21 antibody [HUGO291] (ab107099)](https://www.abcam.com/ps/products/107/ab107099/Images/ab107099-119534-anti-p21-antibody-hugo291-western-blot.jpg)
Western blot - Anti-p21 antibody [HUGO291] (ab107099)All lanes : Anti-p21 antibody [HUGO291] (ab107099) at 1/50 dilution
Lane 1 : TT243 MEFs (non-irradiated) at 18.83 µg
Lane 2 : TT243 MEFs (irradiated with 10 Gy) at 20.8 µg
Lane 3 : p21-/- KO MEFs at 60 µg
Lane 4 : P21 WT MEFs at 75 µg
Lane 5 : HEK-V5-mp21 transfected cells at 20 µg
Lane 6 : HEK-V5-mp15 transfected cells at 20 µg
Predicted band size: 18 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)](https://www.abcam.com/ps/products/107/ab107099/Images/ab107099-266543-anti-p21-antibody-hugo291-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hek-V5-mp21 transfected cells labelling p21 with ab107099. Nuclear staining was observed in Hek-V5-mp21 cytospins. Labeling with the anti-V5 mAb confirmed the efficiency of transfection. Cytospin preparation of human CD8 was used as a negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)](https://www.abcam.com/ps/products/107/ab107099/Images/ab107099-119535-anti-p21-antibody-hugo291-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)ab107099 at 1/100 staining p21 in conditional TRF1 knock out Mouse skin tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)](https://www.abcam.com/ps/products/107/ab107099/Images/ab107099-119533-anti-p21-antibody-hugo291-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (ab107099)ab107099 at 1/100 staining p21 in Mouse skin papilloma tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab107099 has been referenced in 17 publications.
- Feng Q et al. Long non-coding RNA HOTAIR promotes the progression of synovial sarcoma through microRNA-126/stromal cell-derived factor-1 regulation. Oncol Lett 21:444 (2021). PubMed: 33868482
- Lagnado A et al. Neutrophils induce paracrine telomere dysfunction and senescence in ROS-dependent manner. EMBO J 40:e106048 (2021). PubMed: 33764576
- Guo Q et al. STING promotes senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the NF-?B signaling pathway. Cell Death Dis 12:13 (2021). PubMed: 33414452
- Okuyama K et al. Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma. Sci Rep 11:5259 (2021). PubMed: 33664437
- Amrenova A et al. Cell competition between anaplastic thyroid cancer and normal thyroid follicular cells exerts reciprocal stress response defining tumor suppressive effects of normal epithelial tissue. PLoS One 16:e0249059 (2021). PubMed: 33793628