Anti-p21-ARC/ARPC3 antibody (ab96137)

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Overview

  • Product name

    Anti-p21-ARC/ARPC3 antibody
    See all p21-ARC/ARPC3 primary antibodies

  • Description

    Rabbit polyclonal to p21-ARC/ARPC3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish

  • Immunogen

    Recombinant fragment corresponding to Human p21-ARC/ARPC3.
    Database link: NP_005710

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and cell lines. Raji whole cell lysate for WB and endo mitral OVCA tissue for IHC.

  • General notes

     This product was previously labelled as p21-ARC

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).

  • Cellular localization

    Cytoplasmic; cytoskeleton

  • Database links

    see all

  • Alternative names

    • Actin related protein 2/3 complex subunit 3 antibody
    • ARC21 antibody
    • ARP2/3 complex 21 kDa subunit antibody
    • ARP2/3 protein complex subunit p21 antibody
    • ARPC3 antibody
    see all

Images

  • Western blot - Anti-p21-ARC/ARPC3 antibody (ab96137)
    Western blot - Anti-p21-ARC/ARPC3 antibody (ab96137)
    Anti-p21-ARC/ARPC3 antibody (ab96137) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21-ARC/ARPC3 antibody (ab96137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21-ARC/ARPC3 antibody (ab96137)
    ab96137, at 1/100, staining paraffin-embedded endo mitral OVCA by Immunohistochemistry.

References

This product has been referenced in:

  • Leyton-Puig D  et al. Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors. Nat Commun 8:16068 (2017). Read more (PubMed: 28703125) »
See 1 Publication for this product

Publishing research using ab96137? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-p21-ARC/ARPC3 antibody

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell line)
Gel Running Conditions
Reduced Denaturing (12% and 15%)
Loading amount
50 µg
Specification
Breast cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Otto Cerqueira

Verified customer

Submitted Oct 22 2011

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