Anti-p21 (phospho T145) antibody (ab47300)

Thank you for contacting Abcam. I have looked at the two antibodies you are interested in, ab39409 and ab47300 and since neither has been tested in mouse, we cannot gurantee that they will work for your particular requirments. However, what I can offer is our testing discount program. I have placed the details of the program below: As neither ab39409 or 47300 have not been tested in mouse tissue, I can offer a discount off a future purchase if you buy either ab39409 or 47300 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody). If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab39409 or 47300 in mouseI will then send a discount code. This code must be issued before purchasing ab(XXX) so please wait for my reply before ordering. 2. Purchase ab39409 or 47300 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab39409 or 47300 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.    

Thank you for your enquiry. The reason why the detected and expected bands are different is maybe due to modifications like the following: 1) post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein 2) post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases 3) splice variants - alternative splicing may create different sized proteins from the same gene 4) relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands I am afraid that we do not know the exact cause of the difference in band size. The image that we have was the outcome our laboratory obtained during their testing. I am sorry I can't be of more assistance in this occasion but should you require any other information, please do not hesitate to contact me.