• Product name

    Anti-p21 Ras antibody [M337]
  • Description

    Mouse monoclonal [M337] to p21 Ras
  • Host species

  • Specificity

    ab86696 may detect N-Ras and K-Ras.
  • Tested applications

    Suitable for: WB, ICC, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein from human c-H-Ras sequence.

  • Positive control

    • Adult mouse brain, human A431 cell and human Jurkat cell lysates.



Our Abpromise guarantee covers the use of ab86696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 21 kDa.
ICC 1/100.
IHC-P 1/1.
ELISA 1/2000.


  • Relevance

    The human ras gene family consists of three identified members which encode proteins of 21 kDa. Human c-H-ras and c-K-ras are the cellular homologs of v-Hand v-K-ras originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. The third family member is designated c-N-ras. Normal cellular ras genes are referred to as protooncogenes and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61. Such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis remains to be determined.
  • Cellular localization

  • Database links

  • Alternative names

    • C K RAS antibody
    • c K ras protein antibody
    • c K ras2 protein antibody
    • c Ki ras antibody
    • C Kirsten ras protein antibody
    • Cellular c Ki ras2 proto oncogene antibody
    • GTPase KRas antibody
    • GTPase NRas antibody
    • K Ras 2 antibody
    • K ras p21 protein antibody
    • K RAS2A antibody
    • K RAS2B antibody
    • K RAS4A antibody
    • K RAS4B antibody
    • KI RAS antibody
    • Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog antibody
    • KRAS antibody
    • KRAS1 antibody
    • KRAS2 antibody
    • N ras antibody
    • Neuroblastoma RAS viral (v-ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS3 antibody
    • oncogene KRAS2 antibody
    • PR310 c K ras oncogene antibody
    • RASK2 antibody
    • Transforming protein N Ras antibody
    • transforming protein p21 antibody
    • v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all


  • All lanes : Anti-p21 Ras antibody [M337] (ab86696) at 1/500 dilution

    Lane 1 : Mouse brain
    Lane 2 : Human A431 cell lysate
    Lane 3 : Jurkat cell lysate

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • IHC image of ab86696 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86696, undiluted, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Kong YH & Xu SP Salidroside prevents skin carcinogenesis induced by DMBA/TPA in a mouse model through suppression of inflammation and promotion of apoptosis. Oncol Rep 39:2513-2526 (2018). Read more (PubMed: 29693192) »
See 1 Publication for this product

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