Key features and details
- Mouse monoclonal [JJ6] to p23
- Suitable for: WB, ICC/IF, IHC
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-p23 antibody [JJ6]
See all p23 primary antibodies
DescriptionMouse monoclonal [JJ6] to p23
Tested applicationsSuitable for: WB, ICC/IF, IHCmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human p23.
Database link: Q15185
- ICC/IF: HaCaT cells and heat shocked HeLa cells. IHC: Mouse back skin. WB: Mixture of human cell lines, cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab223608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 19 kDa.|
FunctionMolecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.
PathwayLipid metabolism; prostaglandin biosynthesis.
Sequence similaritiesBelongs to the p23/wos2 family.
Contains 1 CS domain.
- Information by UniProt
- Co chaperone p23 antibody
- cPGES antibody
- Cytosolic prostaglandin E synthase antibody
Anti-p23 antibody [JJ6] (ab223608) at 1/1000 dilution + Mixture of human cell lines, cell lysate at 15 µg
Sheep Anti-Mouse IgG: HRP
Predicted band size: 19 kDa
Blocking: 1.5% BSA for 30 minutes at RT.
The cell line mixture contains: A431 (human epidermoid carcinoma cell line), A549 (human lung carcinoma cell line), HCT116 (human colon cancer), HeLa (human epithelial cell line from cervix adenocarcinoma), HEK-293 (human epithelial cell line from embryonic kidney), HepG2 (human liver hepatocellular carcinoma cell line), HL-60 (human promyelocytic leukemia cell line), HUVEC (human umbilical vein endothelial cell line), Jurkat (human T cell leukemia cell line from peripheral blood), MCF7 (human breast adenocarcinoma cell line), PC-3 (human prostate adenocarcinoma cell line) and T98G (human brain glioblastoma).
Bouin's fixed-paraffin embedded mouse back skin tissue stained for p23 with ab223608 (green) (1/100) in immunohistochemical analysis. Secondary antibody: FITC Goat Anti-Mouse at 1/50.
2% formaldehyde fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for p23 (green) using ab223608 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse at 1/200. DAPI counterstain (blue).
100% methanol fixed HaCaT (human keratinocyte cell line) cells stained for p23 (green) using ab223608 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse at 1/50.
ab223608 has not yet been referenced specifically in any publications.