Anti-p27 KIP 1 antibody (ab215434)
Key features and details
- Rabbit polyclonal to p27 KIP 1
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-p27 KIP 1 antibody
See all p27 KIP 1 primary antibodies -
Description
Rabbit polyclonal to p27 KIP 1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human p27 KIP 1 aa 150-198 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:GIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT
Database link: P46527 -
Positive control
- Human ovary carcinoma tissue; MCF-7 cells; rat RSC96 cells; mouse lung lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab215434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/20 - 1/100. | |
| IHC-P | 1/100 - 1/500. When using a fluorescent secondary probe the recommended dilution is 1/50 - 1/200. |
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| WB | 1/100 - 1/1000. Predicted molecular weight: 22 kDa. |
Target
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Function
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. -
Tissue specificity
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. -
Involvement in disease
Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. -
Sequence similarities
Belongs to the CDI family. -
Domain
A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity. -
Post-translational
modificationsPhosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation. -
Cellular localization
Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation. - Information by UniProt
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Database links
- Entrez Gene: 1027 Human
- Entrez Gene: 12576 Mouse
- Entrez Gene: 83571 Rat
- Omim: 600778 Human
- SwissProt: P46527 Human
- SwissProt: P46414 Mouse
- Unigene: 238990 Human
- Unigene: 2958 Mouse
see all -
Alternative names
- AA408329 antibody
- AI843786 antibody
- Cdki1b antibody
see all
Images
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Western blot - Anti-p27 KIP 1 antibody (ab215434)Anti-p27 KIP 1 antibody (ab215434) at 1/300 dilution (overnight at 4°C) + Mouse lung lysates
Secondary
conjugated secondary antibody (60 minutes at 37°C) at 1/10000 dilution
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (ab215434)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovary carcinoma tissue labeling p27 KIP 1 with ab215434 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow Cytometry - Anti-p27 KIP 1 antibody (ab215434)Flow cytometric analysis of rat RSC96 cells labeling p27 KIP 1 with ab215434 at 1/100 dilution for 30 minutes, followed by incubation with a PE conjugated secondary for 30 minutes (green), compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (ab215434)Immunohistochemical analysis of MCF-7 cells labeling p27 KIP 1 with ab215434 at 1/500 dilution in PBS for 2 hours at 37°C, followed by Goat anti-rabbit IgG (H+L), Cy3 conjugated secondary antibody. The nuclei were stained with DAPI.
Protocols
Datasheets and documents
References (5)
ab215434 has been referenced in 5 publications.
- Song X et al. Long Noncoding RNA SOX2-OT Knockdown Inhibits Proliferation and Metastasis of Prostate Cancer Cells Through Modulating miR-452-5p/HMGB3 Axis and Inactivating Wnt/ß-Catenin Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32407168
- Yang Y et al. Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis. Braz J Med Biol Res 52:e8412 (2019). PubMed: 31038581
- Peng F et al. PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. Int J Oncol 52:945-954 (2018). PubMed: 29393396
- Yu Z et al. Bufalin suppresses hepatocarcinogenesis by targeting ß-catenin/TCF signaling via cell cycle-related kinase. Sci Rep 8:3891 (2018). PubMed: 29497076
- Jurisevic M et al. The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis. Oncotarget 9:28195-28212 (2018). PubMed: 29963272
