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    p27-kip-1-antibody-epfhcr16a-ab113075.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Cip / Kip
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)

  • Datasheet
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Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)
  • Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)
  • Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPFHCR16a] to p27 KIP 1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-p27 KIP 1 antibody [EPFHCR16a]
    See all p27 KIP 1 primary antibodies
  • Description

    Rabbit monoclonal [EPFHCR16a] to p27 KIP 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human p27 KIP 1 aa 1 to the C-terminus.
    Database link: P46527

  • Positive control

    • MCF-7 and SH-SY5Y cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPFHCR16a
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Cip / Kip
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Cip/Kip
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Cip/kip

Associated products

  • Alternative Versions

    • Anti-p27 KIP 1 antibody [EPFHCR16a] - BSA and Azide free (ab247947)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)
  • Recombinant Protein

    • Recombinant Human p27 KIP 1 protein (Tagged) (ab56279)

Applications

Our Abpromise guarantee covers the use of ab113075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
    • Tissue specificity

      Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
    • Involvement in disease

      Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
    • Sequence similarities

      Belongs to the CDI family.
    • Domain

      A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.
    • Post-translational
      modifications

      Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
      Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
      Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation.
    • Target information above from: UniProt accession P46527 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1027 Human
      • Omim: 600778 Human
      • SwissProt: P46527 Human
      • Unigene: 238990 Human
      • Alternative names

        • AA408329 antibody
        • AI843786 antibody
        • Cdki1b antibody
        • CDKN 1B antibody
        • CDKN 4 antibody
        • CDKN1B antibody
        • CDKN4 antibody
        • CDN1B_HUMAN antibody
        • Cyclin Dependent Kinase Inhibitor 1B antibody
        • Cyclin dependent kinase inhibitor p27 antibody
        • Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody
        • Cyclin-dependent kinase inhibitor 1B antibody
        • Cyclin-dependent kinase inhibitor p27 antibody
        • Cyclin-dependent kinase inhibitor p27 Kip1 antibody
        • KIP 1 antibody
        • KIP1 antibody
        • MEN1B antibody
        • MEN4 antibody
        • OTTHUMP00000195098 antibody
        • OTTHUMP00000195099 antibody
        • p27 antibody
        • p27 Kip1 antibody
        • P27-like cyclin-dependent kinase inhibitor antibody
        • p27Kip1 antibody
        see all

      Images

      • Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)
        Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)

        ab113075 was shown to specifically react with p27 KIP1 in wild-type HAP1 cells. No band was observed when knock out samples were examined.

      • Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)
        Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)
        All lanes : Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075) at 1/1000 dilution

        Lane 1 : MCF-7 cell lysates
        Lane 2 : SH-SY5Y cell lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 22 kDa

      • Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)
        Anti-p27 KIP 1 antibody [EPFHCR16a] (ab113075)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab113075? Please let us know so that we can cite the reference in this datasheet.

      ab113075 has been referenced in 1 publication.

      • Kim HK  et al. Expression of Proviral Integration Site for Moloney Murine Leukemia Virus 1 (Pim-1) Is Post-transcriptionally Regulated by Tristetraprolin in Cancer Cells. J Biol Chem 287:28770-8 (2012). WB ; Human . PubMed: 22700982

      Customer reviews and Q&As

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