Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (ab212252)
Key features and details
- Mouse monoclonal [KIP1/769] to p27 KIP 1 - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free
See all p27 KIP 1 primary antibodies -
Description
Mouse monoclonal [KIP1/769] to p27 KIP 1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human p27 KIP 1.
Database link: P46527 -
Positive control
- ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa, Jurkat and MCF7 cells; Human tonsil, breast, cervical, prostate and colon carcinoma tissue; Rat colon tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KIP1/769 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.5-1µg for 106 cells.
In 0.1ml. |
|
IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
Flow Cyt
Use 0.5-1µg for 106 cells. In 0.1ml. |
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. -
Tissue specificity
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. -
Involvement in disease
Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. -
Sequence similarities
Belongs to the CDI family. -
Domain
A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity. -
Post-translational
modificationsPhosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation. -
Cellular localization
Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation. - Information by UniProt
-
Database links
- Entrez Gene: 1027 Human
- Entrez Gene: 83571 Rat
- Omim: 600778 Human
- SwissProt: P46527 Human
- Unigene: 238990 Human
-
Alternative names
- AA408329 antibody
- AI843786 antibody
- Cdki1b antibody
see all
Images
-
Flow Cytometry analysis of Jurkat cells labeling p27 Kip1 with ab212252 at 1 µg/mL: Green.
Black: Jurkat cells alone. Grey: Isotype control. -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling p27 Kip1 with ab212252 at 0.5 µg/mL.
-
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate cancer tissue labeling p27 Kip1 with ab212252 at 0.5 µg/mL.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling p27 Kip1 with ab212252 at 0.5 µg/mL.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab212252 has not yet been referenced specifically in any publications.