Anti-p27 KIP 1 antibody [SX53G8] (ab193379)
![Immunocytochemistry - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)](https://www.abcam.com/ps/products/193/ab193379/Images/ab193379-453209-Antip27-KIP-1-antibody-ICC-MCF7.jpg "Immunocytochemistry - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)")
Key features and details
- Mouse monoclonal [SX53G8] to p27 KIP 1
- Suitable for: WB, IHC-P, ICC/IF, IP, IHC-Fr, Flow Cyt, ICC
- Knockout validated
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG1
Overview
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Product name
Anti-p27 KIP 1 antibody [SX53G8]
See all p27 KIP 1 primary antibodies -
Description
Mouse monoclonal [SX53G8] to p27 KIP 1 -
Host species
Mouse -
Specificity
ab193379 is highly specific and shows no cross-reaction with other related mitotic inhibitors. -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IP, IHC-Fr, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Monkey
Predicted to work with: Cat, Dog, Chinese hamster
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Immunogen
Recombinant full length protein (proprietary-tag) corresponding to Human p27 KIP 1 aa 1 to the C-terminus.
Database link: P46527 -
Positive control
- WB: HAP1, HeLa and MCF7 cell lysate. IHC: Human colon tissue. ICC: MCF-7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SX53G8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 22 kDa.
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC |
Use a concentration of 1 - 2 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 22 kDa. |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. -
Tissue specificity
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. -
Involvement in disease
Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. -
Sequence similarities
Belongs to the CDI family. -
Domain
A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity. -
Post-translational
modificationsPhosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation. -
Cellular localization
Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation. - Information by UniProt
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Database links
- Entrez Gene: 493958 Cat
- Entrez Gene: 403429 Dog
- Entrez Gene: 1027 Human
- Entrez Gene: 12576 Mouse
- Entrez Gene: 83571 Rat
- Omim: 600778 Human
- SwissProt: O19001 Cat
- SwissProt: Q60439 Chinese hamster
see all -
Alternative names
- AA408329 antibody
- AI843786 antibody
- Cdki1b antibody
see all
Images
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Immunocytochemistry - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)
Immunocytochemical analysis of PFA-fixed MCF-7 cells labeling p27 KIP 1 with ab193379 at 1 µg/mL. A Goat anti-mouse IgG CF488 antibody (Green) was used as a secondary antibody. The membrane is stained with Phalloidin-CF640
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![Western blot - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)](https://www.abcam.com/ps/products/193/ab193379/Images/ab193379-270759-anti-p27-kip-1-antibody-sx53g8-western-blot.jpg)
Western blot - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: CDKN1B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab193379 observed at 30 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab193379 was shown to specifically recognize CDKN1B in wild-type HAP1 cells as well as additional cross-reactive bands. No bands were observed when CDKN1B knockout samples were used. Wild-type and CDKN1B knockout samples were subjected to SDS-PAGE. ab193379 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/mL and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)](https://www.abcam.com/ps/products/193/ab193379/Images/ab193379-230520-anti-p27-kip-1-antibody-sx53g8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling p27 KIP 1 with ab193379 at 1 µg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab193379 has been referenced in 12 publications.
- Sun L & Zhang X Report of a rare case of congenital mitral valve prolapse with chronic kidney disease--reconsidered genotype-phenotypic correlations. Mol Genet Genomic Med 9:e1558 (2021). PubMed: 33225636
- Zhang Q et al. FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop. Cell Death Dis 12:275 (2021). PubMed: 33723215
- Wu S et al. Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN-PI3K-AKT signalling pathway in gastric cancer. J Cell Mol Med 25:8890-8903 (2021). PubMed: 34382342
- Huang Z et al. miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer. Cell Death Dis 10:372 (2019). PubMed: 31068580
- Yu F et al. Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells. Mol Med Rep 19:4326-4334 (2019). PubMed: 30942392
