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Cell Biology Cell Cycle Cell Cycle Inhibitors Cip / Kip
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Anti-p27 KIP 1 (phospho T198) antibody (ab64949)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (7)

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Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
  • Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)

Key features and details

  • Rabbit polyclonal to p27 KIP 1 (phospho T198)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human p27 KIP 1 protein (Tagged) (ab56279)

View more associated products

Overview

  • Product name

    Anti-p27 KIP 1 (phospho T198) antibody
    See all p27 KIP 1 primary antibodies
  • Description

    Rabbit polyclonal to p27 KIP 1 (phospho T198)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of p27 Kip 1 only when phosphorylated at threonine 198.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 198 (RRRQTP)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Cip / Kip
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Cip/Kip
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Cip/kip

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Hep G2 cytoplasmic extract lysate (ab14659)
    • Hep G2 nuclear extract lysate (ab14660)
  • Recombinant Protein

    • Recombinant Human p27 KIP 1 protein (Tagged) (ab56279)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB (2)
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
Notes
WB
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
  • Tissue specificity

    Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
  • Involvement in disease

    Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
  • Sequence similarities

    Belongs to the CDI family.
  • Domain

    A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.
  • Post-translational
    modifications

    Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
    Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
    Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation.
  • Target information above from: UniProt accession P46527 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1027 Human
    • Omim: 600778 Human
    • SwissProt: P46527 Human
    • Unigene: 238990 Human
    • Alternative names

      • AA408329 antibody
      • AI843786 antibody
      • Cdki1b antibody
      • CDKN 1B antibody
      • CDKN 4 antibody
      • CDKN1B antibody
      • CDKN4 antibody
      • CDN1B_HUMAN antibody
      • Cyclin Dependent Kinase Inhibitor 1B antibody
      • Cyclin dependent kinase inhibitor p27 antibody
      • Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody
      • Cyclin-dependent kinase inhibitor 1B antibody
      • Cyclin-dependent kinase inhibitor p27 antibody
      • Cyclin-dependent kinase inhibitor p27 Kip1 antibody
      • KIP 1 antibody
      • KIP1 antibody
      • MEN1B antibody
      • MEN4 antibody
      • OTTHUMP00000195098 antibody
      • OTTHUMP00000195099 antibody
      • p27 antibody
      • p27 Kip1 antibody
      • P27-like cyclin-dependent kinase inhibitor antibody
      • p27Kip1 antibody
      see all

    Images

    • Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
      Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
      All lanes : Anti-p27 KIP 1 (phospho T198) antibody (ab64949) at 1/500 dilution

      Lane 1 : HeLa cell extract
      Lane 2 : HepG2 cell extract
      Lane 3 : COLO205 cell extract
      Lane 4 : COLO205 cell extract with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 22 kDa
      Observed band size: 28 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
      Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)This image is courtesy of an anonymous Abreview
      Anti-p27 KIP 1 (phospho T198) antibody (ab64949) at 1/1000 dilution + Mouse liver tissue lysate at 40 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 22 kDa
      Observed band size: 34 kDa why is the actual band size different from the predicted?


      Exposure time: 1 second

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab64949? Please let us know so that we can cite the reference in this datasheet.

    ab64949 has been referenced in 7 publications.

    • Ng PK  et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell 33:450-462.e10 (2018). PubMed: 29533785
    • Hill SM  et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst 4:73-83.e10 (2017). PubMed: 28017544
    • Kim JA  et al. Comprehensive functional analysis of the tousled-like kinase 2 frequently amplified in aggressive luminal breast cancers. Nat Commun 7:12991 (2016). WB . PubMed: 27694828
    • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). PubMed: 27274057
    • Lohmann F  et al. UCH-L1 induces podocyte hypertrophy in membranous nephropathy by protein accumulation. Biochim Biophys Acta 1842:945-58 (2014). PubMed: 24583340
    • Liu R  et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10:1241-55 (2014). PubMed: 24905460
    • Inge LJ  et al. Dasatinib, a small molecule inhibitor of the Src kinase, reduces the growth and activates apoptosis in pre-neoplastic Barrett's esophagus cell lines: evidence for a noninvasive treatment of high-grade dysplasia. J Thorac Cardiovasc Surg 145:531-8 (2013). Human . PubMed: 23142123

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-p27 KIP 1 (phospho T198) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 14 2017

    Western blot abreview for Anti-p27 KIP 1 (phospho T198) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 02 2014

    Western blot abreview for Anti-p27 KIP 1 (phospho T198) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Loading amount
    80 µg
    Specification
    MEFs
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 02 2008

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