Overview

  • Product name
  • Description
    Rabbit polyclonal to P2X3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Non human primates
  • Immunogen

    Synthetic peptide:

    VEKQSTDSGAYSIGH

    , corresponding to amino acids 383-397 of C-terminus of Rat P2X3.

Properties

Applications

Our Abpromise guarantee covers the use of ab10269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 47.6 kDa.
IHC-Fr 1/1000.
ICC 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
IHC-FoFr Use at an assay dependent concentration.

Target

Images

  • Image A: ab10248, at a dilution of 1/1000, staining P2X3 (green) in rat dorsal root ganglia sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Other structures visualized with anti-ASIC3 (red). Image B: Structures in rat dorsal root ganglia sections visualized with anti-ASIC3 (red) and anti-TRPV1 (green). Double-labeled cells are yellow or orange.
  • ICC/IF image of ab10269 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10269, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Ding S  et al. P2X3 receptor involvement in endometriosis pain via ERK signaling pathway. PLoS One 12:e0184647 (2017). Read more (PubMed: 28898282) »
  • Weng ZJ  et al. Effect of electroacupuncture on P2X3 receptor regulation in the peripheral and central nervous systems of rats with visceral pain caused by irritable bowel syndrome. Purinergic Signal 11:321-9 (2015). Read more (PubMed: 25809868) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for contacting us regarding anti-P2X3 antibody (ab10269).


Unfortunately , as discussed over the phone, the exact concentration of ab10269 has not been determined. This is quite common with antibody preparations which are supplied as unpurified whole antiserum. I can however tell you that this antibody is estimated to have a concentration of 1 mg/mL.


I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Question
Answer

Thank you for contacting us.

The antibody is a whole anti serum, specifc IgG is not purified so we do not know the conc. of this antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal cord lysate)
Loading amount
40 µg
Specification
Spinal cord lysate
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Richard D'Mello

Verified customer

Submitted Jun 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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