Key features and details
- Goat polyclonal to P2X4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P2X4 antibody
See all P2X4 primary antibodies
DescriptionGoat polyclonal to P2X4
Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver in Western blot.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Orangutan
- WB: A549 cell lysate; ICC/IF: A431 cells; IHC-P: Human uterus tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab134559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48-50 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product.
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionReceptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Sequence similaritiesBelongs to the P2X receptor family.
- Information by UniProt
- AI504491 antibody
- ATP gated cation channel protein antibody
- ATP receptor antibody
Anti-P2X4 antibody (ab134559) at 0.3 µg/ml + A549 (human lung carcinoma cell line) cell lysate (35µg protein in RIPA buffer)
Predicted band size: 43 kDa
Observed band size: 48-50 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of paraformaldehyde fixed A431 cells labeling P2X4 with ab134559. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (4 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (4 µg/mL).
ab134559 has been referenced in 1 publication.
- Asif A et al. Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: an approach towards proinflammatory role of P2X4 and P2X7 receptors. Purinergic Signal 15:367-374 (2019). PubMed: 31401785