Key features and details
- Mouse polyclonal to P2X4
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P2X4 antibody
See all P2X4 primary antibodies
DescriptionMouse polyclonal to P2X4
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Full length protein corresponding to Human P2X4 aa 1-388.
MAGCCAALAAFLFEYDTPRIVLIRSRKVGLMNRAVQLLILAYVIGWVFVW EKGYQETDSVVSSVTTKVKGVAVTNTSKLGFRIWDVADYVIPAQEENSLF VMTNVILTMNQTQGLCPEIPDATTVCKSDASCTAGSAGTHSNGVSTGRCV AFNGSVKTCEVAAWCPVEDDTHVPQPAFLKAAENFTLLVKNNIWYPKFNF SKRNILPNITTTYLKSCIYDAKTDPFCPIFRLGKIVENAGHSFQDMAVEG GIMGIQVNWDCNLDRAASLCLPRYSFRRLDTRDVEHNVSPGYNFRFAKYY RDLAGNEQRTLIKAYGIRFDIIVFGKAGKFDIIPTMINIGSGLALLGMAT VLCDIIVLYCMKKRLYYREKKYKYVEDYEQGLASELDQ
Database link: NP_002551.2
- P2X4 transfected 293T cell lysate; P2X4 expressing 293 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab168939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Sequence similaritiesBelongs to the P2X receptor family.
- Information by UniProt
- AI504491 antibody
- ATP gated cation channel protein antibody
- ATP receptor antibody
Flow cytometric analysis of negative control 293 cells (Black) and P2X4 expressing 293 cells (Green) labeling P2X4 with ab168939 at 1 ug/ml.
All lanes : Anti-P2X4 antibody (ab168939) at 1 µg/ml
Lane 1 : P2X4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
ab168939 has been referenced in 2 publications.
- Dave KM et al. Characterization of the SIM-A9 cell line as a model of activated microglia in the context of neuropathic pain. PLoS One 15:e0231597 (2020). PubMed: 32287325
- Chatterjee C & Sparks DL P2X receptors regulate adenosine diphosphate release from hepatic cells. Purinergic Signal 10:587-93 (2014). PubMed: 25059924