Key features and details
- Rabbit polyclonal to P2X4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P2X4 antibody
See all P2X4 primary antibodies
DescriptionRabbit polyclonal to P2X4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human placenta, cerebral cortex and small intestine tissue. WB: SK-MEL-30 cell lysate. ICC/IF: U-2 OS cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionReceptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Sequence similaritiesBelongs to the P2X receptor family.
- Information by UniProt
- AI504491 antibody
- ATP gated cation channel protein antibody
- ATP receptor antibody
PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling P2X4 using ab243734 at 4 µg/ml (green) in ICC/IF.
Anti-P2X4 antibody (ab243734) at 0.4 µg/ml + SK-MEL-30 cell lysate
Predicted band size: 43 kDa
Formalin-fixed, paraffin-embedded human small intestine tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human placenta tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis. No membranous positivity in myocytes, as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243734 has not yet been referenced specifically in any publications.