Key features and details
- Goat polyclonal to P2X4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77434 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Sequence similaritiesBelongs to the P2X receptor family.
- Information by UniProt
- AI504491 antibody
- ATP gated cation channel protein antibody
- ATP receptor antibody
All lanes : Anti-P2X4 antibody (ab77434) at 1 µg/ml
Lane 1 : Human kidney lysate (in RIPA buffer)
Lane 2 : Human liver lysate (in RIPA buffer)
Lane 3 : Rat kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Detected by chemiluminescence.
ab77434 has not yet been referenced specifically in any publications.