Overview

  • Product name
  • Description
    Goat polyclonal to P2X4
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human P2X4 (C terminal).
    Sequence:

    C-YREKKYKYVEDYEQ


    Database link: NP_002551.2

  • Positive control
    • Human kidney lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: antibody detection limit dilution: 1/32000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • All lanes : Anti-P2X4 antibody (ab77434) at 1 µg/ml

      Lane 1 : Human kidney lysate (in RIPA buffer)
      Lane 2 : Human liver lysate (in RIPA buffer)
      Lane 3 : Rat kidney lysate (in RIPA buffer)

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 43 kDa
      Observed band size: 60 kDa (why is the actual band size different from the predicted?)



      Primary incubation was 1 hour.

      Detected by chemiluminescence.

    References

    ab77434 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77434.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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