Key features and details
- Rat monoclonal [1F11] to P2X7
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2b
Product nameAnti-P2X7 antibody [1F11]
See all P2X7 primary antibodies
DescriptionRat monoclonal [1F11] to P2X7
Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse P2X7. (Mouse mast cells sorted from colon).
- Flow Cyt: C57BL6 mouse splenocytes IHC-Fr: Mouse Large Intestine
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab195356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 10 µg/ml.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
FunctionReceptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Tissue specificityWidely expressed with highest levels in brain and immune tissues.
Sequence similaritiesBelongs to the P2X receptor family.
modificationsPhosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
Cellular localizationCell membrane.
- Information by UniProt
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (ab18541) (left) or anti-P2X7 [F11] antibody (ab233698) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (ab190299).
Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes
IHC image of ab195356 staining in 10% formaldehyde fixed frozen tissue section of normal mouse large intestine.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab195356 (1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150167 (Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab195356.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab195356 has been referenced in 1 publication.
- Kurashima Y et al. Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors. Nat Commun 3:1034 (2012). Functional Studies, Flow Cyt ; Mouse . PubMed: 22948816