Overview

  • Product name

    Anti-P2X7 antibody [1F11]
    See all P2X7 primary antibodies
  • Description

    Rat monoclonal [1F11] to P2X7
  • Host species

    Rat
  • Tested applications

    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse P2X7. (Mouse mast cells sorted from colon).

  • Positive control

    • Flow Cyt: C57BL6 mouse splenocytes IHC-Fr: Mouse Large Intestine
  • General notes

    This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1F11
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 5 µg/ml.

Target

  • Function

    Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
  • Tissue specificity

    Widely expressed with highest levels in brain and immune tissues.
  • Sequence similarities

    Belongs to the P2X receptor family.
  • Post-translational
    modifications

    Phosphorylation results in its inactivation.
    ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
    Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP receptor antibody
    • P2rx7 antibody
    • P2RX7_HUMAN antibody
    • P2X purinoceptor 7 antibody
    • P2X7 antibody
    • P2Z receptor antibody
    • Purinergic receptor antibody
    • purinergic receptor P2X, ligand gated ion channel, 7 antibody
    see all

Images

  • C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (ab18541) (left) or anti-P2X7 [F11] antibody (ab233698) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (ab190299).

    Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes

  • IHC image of ab195356 staining in 10% formaldehyde fixed frozen tissue section of normal mouse large intestine.

    Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab195356 (1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150167 (Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab195356. 

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

References

This product has been referenced in:

  • Kurashima Y  et al. Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors. Nat Commun 3:1034 (2012). Functional Studies, Flow Cyt ; Mouse . Read more (PubMed: 22948816) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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