Key features and details
- Rabbit polyclonal to P2X7
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-P2X7 antibody
See all P2X7 primary antibodies
DescriptionRabbit polyclonal to P2X7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human P2X7 aa 47-334. Expressed in E. coli. N-terminal tags.
SDKLYQRKEPVISSVHTKVKGIAEVKEEIVENGVKKLVHSVFDTADYTFP LQGNSFFVMTNFLKTEGQEQRLCPEYPTRRTLCSSDRGCKKGWMDPQSKG IQTGRCVVYEGNQKTCEVSAWCPIEAVEEAPRPALLNSAENFTVLIKNNI DFPGHNYTTRNILPGLNITCTFHKTQNPQCPIFRLGDIFRETGDNFSDVA IQGGIMGIEIYWDCNLDRWFHHCRPKYSFRRLDDKTTNVSLYPGYNFRYA KYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLV
Database link: Q99572
- WB: Recombinant human P2X7 protein; Pig brain, stomach and heart tissue. IHC-P: Human kidney tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionReceptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Tissue specificityWidely expressed with highest levels in brain and immune tissues.
Sequence similaritiesBelongs to the P2X receptor family.
modificationsPhosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
Cellular localizationCell membrane.
- Information by UniProt
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for P2X7 using ab233527 at 20 μg/ml in immunohistochemical analysis.
All lanes : Anti-P2X7 antibody (ab233527) at 3 µg/ml
Lane 1 : Pig brain tissue lysate
Lane 2 : Pig stomach tissue lysate
Lane 3 : Pig heart tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Anti-P2X7 antibody (ab233527) at 3 µg/ml + Recombinant human P2X7 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 69 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233527 has not yet been referenced specifically in any publications.