Anti-P2X7 antibody (ab233527)
Key features and details
- Rabbit polyclonal to P2X7
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-P2X7 antibody
See all P2X7 primary antibodies -
Description
Rabbit polyclonal to P2X7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human P2X7 aa 1-350. Expressed in E. coli. N-terminal tags.
Database link: Q99572 -
Positive control
- WB: Recombinant human P2X7 protein; Pig brain, stomach and heart tissue. IHC-P: Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. -
Tissue specificity
Widely expressed with highest levels in brain and immune tissues. -
Sequence similarities
Belongs to the P2X receptor family. -
Post-translational
modificationsPhosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 5027 Human
- Omim: 602566 Human
- SwissProt: Q99572 Human
- Unigene: 729169 Human
-
Alternative names
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for P2X7 using ab233527 at 20 μg/ml in immunohistochemical analysis.
-
All lanes : Anti-P2X7 antibody (ab233527) at 3 µg/ml
Lane 1 : Pig brain tissue lysate
Lane 2 : Pig stomach tissue lysate
Lane 3 : Pig heart tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 69 kDa -
Anti-P2X7 antibody (ab233527) at 3 µg/ml + Recombinant human P2X7 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab233527 has not yet been referenced specifically in any publications.