Product nameAnti-P2X7 antibody
See all P2X7 primary antibodies
DescriptionRabbit polyclonal to P2X7
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Jurkat cell lysate; Human kidney tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab48871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:312500
FunctionReceptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Tissue specificityWidely expressed with highest levels in brain and immune tissues.
Sequence similaritiesBelongs to the P2X receptor family.
modificationsPhosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
Cellular localizationCell membrane.
- Information by UniProt
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
Anti-P2X7 antibody (ab48871) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Antibody diluted in 5% skim milk / PBS buffer; Gel concentration 12%
Ab48871 at 4.0µg/ml staining paraffin embedded human kidney. Magnification x400. Arrows indicate staining of epithelial cells of renal tubule.
This product has been referenced in:
- Yang R et al. Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter. Nat Commun 9:2143 (2018). Read more (PubMed: 29858571) »
- Arima Y et al. Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit. Elife 6:N/A (2017). Read more (PubMed: 28809157) »