Overview

  • Product name

  • Description

    Goat polyclonal to P2X7
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Dog
  • Immunogen

    Synthetic peptide corresponding to Human P2X7 aa 13-26 (N terminal) (Cysteine residue).
    Sequence:

    YETNKVTRIQSMNY

  • Positive control

    • Human brain frontal cortex lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37, 70 kDa (predicted molecular weight: 69 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

Target

  • Function

    Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
  • Tissue specificity

    Widely expressed with highest levels in brain and immune tissues.
  • Sequence similarities

    Belongs to the P2X receptor family.
  • Post-translational
    modifications

    Phosphorylation results in its inactivation.
    ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
    Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP receptor antibody
    • P2rx7 antibody
    • P2RX7_HUMAN antibody
    • P2X purinoceptor 7 antibody
    • P2X7 antibody
    • P2Z receptor antibody
    • Purinergic receptor antibody
    • purinergic receptor P2X, ligand gated ion channel, 7 antibody
    see all

Images

  • All lanes : Anti-P2X7 antibody (ab93354) at 0.3 µg/ml

    Lane 1 : Human brain frontal cortex lysate
    Lane 2 : Human brain frontal cortex lysate with Immunising peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 69 kDa
    Observed band size: 37,70 kDa
    why is the actual band size different from the predicted?

  • ab87597 at 3.8 µg/ml staining P2X7 in Human brain cortex. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

References

This product has been referenced in:

  • Cabahug-Zuckerman P  et al. Potential role for a specialized ß3 integrin-based structure on osteocyte processes in bone mechanosensation. J Orthop Res 36:642-652 (2018). Read more (PubMed: 29087614) »
  • Liu J  et al. Hypermethylation downregulates P2X7receptor expression in astrocytoma. Oncol Lett 14:7699-7704 (2017). Read more (PubMed: 29344216) »
See all 3 Publications for this product

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