Key features and details
- Goat polyclonal to P2X7
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P2X7 antibody
See all P2X7 primary antibodies
DescriptionGoat polyclonal to P2X7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Dog
- Human brain frontal cortex lysate
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab93354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37, 70 kDa (predicted molecular weight: 69 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 6 µg/ml.|
FunctionReceptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Tissue specificityWidely expressed with highest levels in brain and immune tissues.
Sequence similaritiesBelongs to the P2X receptor family.
modificationsPhosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
Cellular localizationCell membrane.
- Information by UniProt
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
All lanes : Anti-P2X7 antibody (ab93354) at 0.3 µg/ml
Lane 1 : Human brain frontal cortex lysate
Lane 2 : Human brain frontal cortex lysate with Immunising peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 37,70 kDa why is the actual band size different from the predicted?
ab87597 at 3.8 µg/ml staining P2X7 in Human brain cortex. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.
ab93354 has been referenced in 4 publications.
- Zhang Q et al. The ATP-P2X7 Signaling Pathway Participates in the Regulation of Slit1 Expression in Satellite Glial Cells. Front Cell Neurosci 13:420 (2019). PubMed: 31607866
- Cabahug-Zuckerman P et al. Potential role for a specialized ß3 integrin-based structure on osteocyte processes in bone mechanosensation. J Orthop Res 36:642-652 (2018). PubMed: 29087614
- Liu J et al. Hypermethylation downregulates P2X7receptor expression in astrocytoma. Oncol Lett 14:7699-7704 (2017). PubMed: 29344216
- Zhang PA et al. Neonatal Maternal Deprivation Enhances Presynaptic P2X7 Receptor Transmission in Insular Cortex in an Adult Rat Model of Visceral Hypersensitivity. CNS Neurosci Ther 23:145-154 (2017). PubMed: 27976523