Anti-P2Y11 antibody - C-terminal (ab183096)

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Western blot - Anti-P2Y11 antibody - C-terminal (ab183096)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y11 antibody - C-terminal (ab183096)

Key features and details

  • Rabbit polyclonal to P2Y11 - C-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-P2Y11 antibody - C-terminal
    See all P2Y11 primary antibodies

  • Description

    Rabbit polyclonal to P2Y11 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human P2Y11 aa 361-374 (C terminal). The exact sequence is proprietary. (NP_002557).
    Sequence:

    APKPSEPQSRELSQ


    Database link: Q96G91
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Hela and 293T cell lysates; Human lung cancer tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/200 - 1/1000. Predicted molecular weight: 40 kDa.

Target

  • Function

    Receptor for ATP and ADP coupled to G-proteins that activate both phosphatidylinositol-calcium and adenylyl cyclase second messenger systems. Not activated by UTP or UDP.

  • Tissue specificity

    Highest expression in liver and spleen.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • P2RY11 antibody
      • P2Y purinoceptor 11 antibody
      • P2Y11 antibody
      • P2Y11 receptor antibody
      • P2Y11_HUMAN antibody
      • Purinergic receptor P2Y G protein coupled 11 antibody
      • Purinergic receptor P2Y11 antibody
      see all

    Images

    • Western blot - Anti-P2Y11 antibody - C-terminal (ab183096)
      Western blot - Anti-P2Y11 antibody - C-terminal (ab183096)
      All lanes : Anti-P2Y11 antibody - C-terminal (ab183096) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 40 kDa


      Exposure time: 1 minute
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y11 antibody - C-terminal (ab183096)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y11 antibody - C-terminal (ab183096)

      Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling P2Y11 with ab183096 at 1/40 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.

    References (1)

    Publishing research using ab183096? Please let us know so that we can cite the reference in this datasheet.

    ab183096 has been referenced in 1 publication.

    • Dreisig K  et al. Human P2Y11 Expression Level Affects Human P2X7 Receptor-Mediated Cell Death. Front Immunol 9:1159 (2018). PubMed: 29937766

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