Overview

  • Product name

    Anti-P2Y12 antibody [1C2A9]
    See all P2Y12 primary antibodies
  • Description

    Mouse monoclonal [1C2A9] to P2Y12
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human P2Y12. The exact sequence is proprietary. Expressed in E.coli.
    Database link: Q9H244

  • Positive control

    • WB: Recombinant human P2Y12 protein; Human P2Y12-hIgG-Fc-transfected HEK-293 cell lysate; PC-3 and C6 cell lysates. Flow cyt: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab233760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation.
  • Tissue specificity

    Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain.
  • Involvement in disease

    Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP glucose receptor antibody
    • ADP-glucose receptor antibody
    • ADPG R antibody
    • ADPG-R antibody
    • ADPGR antibody
    • BDPLT8 antibody
    • G protein coupled receptor SP1999 antibody
    • Gi coupled ADP receptor HORK 3 antibody
    • Gi coupled ADP receptor HORK3 antibody
    • HORK 3 antibody
    • HORK3 antibody
    • P2RY 12 antibody
    • P2RY12 antibody
    • P2T(AC) antibody
    • P2Y 12 antibody
    • P2Y purinoceptor 12 antibody
    • P2Y(12)R antibody
    • P2Y(AC) antibody
    • P2Y(ADP) antibody
    • P2Y(cyc) antibody
    • P2Y12 antibody
    • P2Y12 platelet ADP receptor antibody
    • P2Y12_HUMAN antibody
    • Platelet ADP receptor antibody
    • Purinergic receptor P2RY12 antibody
    • Purinergic receptor P2Y G protein coupled 12 antibody
    • Purinergic receptor P2Y12 antibody
    • Putative G protein coupled receptor antibody
    • SP 1999 antibody
    • SP1999 antibody
    see all

Images

  • Anti-P2Y12 antibody [1C2A9] (ab233760) at 1/500 dilution + Recombinant human P2Y12 protein

    Predicted band size: 39 kDa



    Expected MW is 9.4 kDa.

  • All lanes : Anti-P2Y12 antibody [1C2A9] (ab233760) at 1/500 dilution

    Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Human P2Y12-hIgG-Fc-transfected HEK-293 cell lysate

    Predicted band size: 39 kDa

  • All lanes : Anti-P2Y12 antibody [1C2A9] (ab233760) at 1/500 dilution

    Lane 1 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 2 : C6 (rat glioma cell line) cell lysate

    Predicted band size: 39 kDa

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling P2Y12 with ab233760 at 1/200 dilution (green) compared with a negative control (red).

References

ab233760 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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