Overview

  • Product name

  • Description

    Rabbit polyclonal to P2Y12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human P2Y12 aa 11-24 (N terminal). The exact sequence is proprietary. Genbank: NP_073625
    Sequence:

    PGNTSLCTRDYKIT


    Database link: Q9H244

  • Positive control

    • Human 293T and HeLa whole cell lysate (ab150035); Human lung cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab183066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400 - 1/2000. Predicted molecular weight: 39 kDa.
IHC-P 1/25 - 1/100.

Target

  • Function

    Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation.
  • Tissue specificity

    Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain.
  • Involvement in disease

    Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP glucose receptor antibody
    • ADP-glucose receptor antibody
    • ADPG R antibody
    • ADPG-R antibody
    • ADPGR antibody
    • BDPLT8 antibody
    • G protein coupled receptor SP1999 antibody
    • Gi coupled ADP receptor HORK 3 antibody
    • Gi coupled ADP receptor HORK3 antibody
    • HORK 3 antibody
    • HORK3 antibody
    • P2RY 12 antibody
    • P2RY12 antibody
    • P2T(AC) antibody
    • P2Y 12 antibody
    • P2Y purinoceptor 12 antibody
    • P2Y(12)R antibody
    • P2Y(AC) antibody
    • P2Y(ADP) antibody
    • P2Y(cyc) antibody
    • P2Y12 antibody
    • P2Y12 platelet ADP receptor antibody
    • P2Y12_HUMAN antibody
    • Platelet ADP receptor antibody
    • Purinergic receptor P2RY12 antibody
    • Purinergic receptor P2Y G protein coupled 12 antibody
    • Purinergic receptor P2Y12 antibody
    • Putative G protein coupled receptor antibody
    • SP 1999 antibody
    • SP1999 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling P2Y12 with ab183066 at 1/25 dilution, in the presence (right image) or absence (left image) of immunizing peptide. 

  • All lanes : Anti-P2Y12 antibody (ab183066) at 1/400 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa


    Exposure time: 30 seconds

References

ab183066 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - .01% Triton
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Emily Valued Customer

Verified customer

Submitted Jul 27 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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