Overview

  • Product name

    Anti-P2Y12 antibody [EPR18611]
    See all P2Y12 primary antibodies
  • Description

    Rabbit monoclonal [EPR18611] to P2Y12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse P2Y12 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9CPV9

  • Positive control

    • WB: Rat cerebral cortex, c57 cerebral cortex, rat platelet and Human fetal brain lysates; HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat brain lysates. IP: Rat brain whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/80.
WB 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation.
  • Tissue specificity

    Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain.
  • Involvement in disease

    Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP glucose receptor antibody
    • ADP-glucose receptor antibody
    • ADPG R antibody
    • ADPG-R antibody
    • ADPGR antibody
    • BDPLT8 antibody
    • G protein coupled receptor SP1999 antibody
    • Gi coupled ADP receptor HORK 3 antibody
    • Gi coupled ADP receptor HORK3 antibody
    • HORK 3 antibody
    • HORK3 antibody
    • P2RY 12 antibody
    • P2RY12 antibody
    • P2T(AC) antibody
    • P2Y 12 antibody
    • P2Y purinoceptor 12 antibody
    • P2Y(12)R antibody
    • P2Y(AC) antibody
    • P2Y(ADP) antibody
    • P2Y(cyc) antibody
    • P2Y12 antibody
    • P2Y12 platelet ADP receptor antibody
    • P2Y12_HUMAN antibody
    • Platelet ADP receptor antibody
    • Purinergic receptor P2RY12 antibody
    • Purinergic receptor P2Y G protein coupled 12 antibody
    • Purinergic receptor P2Y12 antibody
    • Putative G protein coupled receptor antibody
    • SP 1999 antibody
    • SP1999 antibody
    see all

Images

  • All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/5000 dilution

    Lane 1 : Rat cerebral cortex lysate
    Lane 2 : c57 cerebral cortex lysate
    Lane 3 : Rat platelet lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • P2Y12 was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab184411 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab184411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: Rat brain whole cell lysate 10µg (Input).
    Lane 2: ab184411 IP in Rat brain whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184411 in Rat brain whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/5000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Xiao M  et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). Read more (PubMed: 30710735) »
  • Guo J  et al. Involvement of P2Y12 receptor of stellate ganglion in diabetic cardiovascular autonomic neuropathy. Purinergic Signal 14:345-357 (2018). Read more (PubMed: 30084083) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain Tissue)
Permeabilization
Yes - 0.02% Triton
Specification
Brain Tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 03 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 19 2016

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