Overview

  • Product name

    Anti-P2Y12 antibody - Extracellular domain
    See all P2Y12 primary antibodies
  • Description

    Rabbit polyclonal to P2Y12 - Extracellular domain
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except CACNA1F (56%).
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Human P2Y12. The peptide corresponds to 16 amino acids from the 2nd extracellular domain of human P2Y12
    Database link: Q9H244

  • Positive control

    • Human bone marrow megakaryocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab188968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation.
  • Tissue specificity

    Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain.
  • Involvement in disease

    Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP glucose receptor antibody
    • ADP-glucose receptor antibody
    • ADPG R antibody
    • ADPG-R antibody
    • ADPGR antibody
    • BDPLT8 antibody
    • G protein coupled receptor SP1999 antibody
    • Gi coupled ADP receptor HORK 3 antibody
    • Gi coupled ADP receptor HORK3 antibody
    • HORK 3 antibody
    • HORK3 antibody
    • P2RY 12 antibody
    • P2RY12 antibody
    • P2T(AC) antibody
    • P2Y 12 antibody
    • P2Y purinoceptor 12 antibody
    • P2Y(12)R antibody
    • P2Y(AC) antibody
    • P2Y(ADP) antibody
    • P2Y(cyc) antibody
    • P2Y12 antibody
    • P2Y12 platelet ADP receptor antibody
    • P2Y12_HUMAN antibody
    • Platelet ADP receptor antibody
    • Purinergic receptor P2RY12 antibody
    • Purinergic receptor P2Y G protein coupled 12 antibody
    • Purinergic receptor P2Y12 antibody
    • Putative G protein coupled receptor antibody
    • SP 1999 antibody
    • SP1999 antibody
    see all

Images

  • Flow Cytometry analysis of mouse primary spinal cord microglia cells labeling P2Y12 with ab188968 at 1/300 dilution. Cells were fixed in paraformaldehyde. Single, live cells were gated on CD45. Cells were incubated with Anti-P2Y12 antibody - Extracellular domain (ab188968) for 15 minutes at 24°C in HBSS, 0.4%BSA, 15mM HEPES, 2mM EDTA buffer. An undiluted allophycocyanin crosslinked Goat Anti-Rabbit IgG secondary antibody was used.

    See Abreview

  • Immunohistochemistry of formalin-fixed, paraffin-embedded Human bone marrow megakaryocytes labelling P2Y12 with ab188968 at 10 µg/ml.

References

ab188968 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (Primary spinal cord microglia)
Permeabilization
No
Gating Strategy
single, live cells were gated on CD45 (all immune cells)
Specification
Primary spinal cord microglia
Preparation
Cell harvesting/tissue preparation method: Percoll gradient isolation from PBS-perfused spinal cord
Sample buffer: HBSS, 0.4%BSA, 15mM HEPES, 2mM EDTA
Fixation
Paraformaldehyde

Dr. Franziska Denk

Verified customer

Submitted Jan 16 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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