The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
WB: 1/20000 - 1/100000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Belongs to the G-protein coupled receptor 1 family.