Product nameAnti-P2Y6 antibody [EPR3816]
See all P2Y6 primary antibodies
DescriptionRabbit monoclonal [EPR3816] to P2Y6
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human P2Y6 (UniProt ID: Q15077).
- A549, HACAT, HepG2, and Human spleen lysates; Human kidney tissue, Human liver tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
WB: 1/20000 - 1/100000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- G coupled nucleotide receptor antibody
- G protein coupled 6 antibody
- MGC15335 antibody
All lanes : Anti-P2Y6 antibody [EPR3816] (ab92504) at 1/20000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue (left-hand image) and Human liver tissue (right-hand image) using ab92504 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.