Key features and details
- Rabbit polyclonal to p35
- Suitable for: IHC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-p35 antibody
See all p35 primary antibodies
DescriptionRabbit polyclonal to p35
Tested applicationsSuitable for: IHC, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Human p35 overexpressing HEK-293T lysate, C-terminal DDK tag. Mouse brain lysate. IHC-P: Mouse brain tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
Functionp35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
Tissue specificityBrain and neuron specific.
Sequence similaritiesBelongs to the cyclin-dependent kinase 5 activator family.
modificationsThe p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
Cellular localizationCell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
- Information by UniProt
- CD5R1_HUMAN antibody
- CDK5 activator 1 antibody
- CDK5P35 antibody
Mouse brain tissue stained for p35 using ab235271 at 1/100 dilution in immunohistochemical analysis. Counterstained with hematoxylin.
HIER antigen retrieval.
ab235271 developed a specific staining of neurons and neurophil in brain tissue.
Anti-p35 antibody (ab235271) at 1/1000 dilution + Mouse brain lysate at 35 µg
Donkey anti Rabbit IgG peroxidase conjugate at 1/70000 dilution
Predicted band size: 34 kDa
Exposure time: 10 seconds
All lanes : Anti-p35 antibody (ab235271) at 1/1000 dilution
Lane 1 : Human CDK5 overexpressing HEK293T lysate
Lane 2 : Human CDK5 empty vector HEK293T lysate
Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) lysate
All lanes : Goat-anti-Rabbit IgG [H&L] at 1/70000 dilution
Predicted band size: 34 kDa
Exposure time: 30 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235271 has not yet been referenced specifically in any publications.