- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-p35 antibody
See all p35 primary antibodies -
DescriptionRabbit polyclonal to p35
-
Host speciesRabbit
-
SpecificityDetects endogenous levels of total p35 protein
-
Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
-
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
-
Immunogen
Synthetic peptide (Human) from an internal sequence
-
Positive control
- Rat brain cell extract HeLa cells
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab64960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). | |
| ICC/IF | 1/500 - 1/1000. | |
| ELISA | 1/20000. |
Target
-
Functionp35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
-
Tissue specificityBrain and neuron specific.
-
Sequence similaritiesBelongs to the cyclin-dependent kinase 5 activator family.
-
Post-translational
modificationsThe p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35. -
Cellular localizationCell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
- Information by UniProt
-
Database links
- Entrez Gene: 8851 Human
- Entrez Gene: 12569 Mouse
- Entrez Gene: 116671 Rat
- Omim: 603460 Human
- SwissProt: Q15078 Human
- SwissProt: P61809 Mouse
- SwissProt: P61810 Rat
- Unigene: 500015 Human
see all -
Alternative names
- CD5R1_HUMAN antibody
- CDK5 activator 1 antibody
- CDK5P35 antibody
see all
Images
-
Western blot - Anti-p35 antibody (ab64960)All lanes : Anti-p35 antibody (ab64960) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 34 kDa
Observed band size: 40 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (ab64960)Immunofluorescence analysis of HeLa cells, using ab64960 at 1/500 dilution. Right hand panel was also treated with immunising peptide.
References
ab64960 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"