Anti-p35 antibody (ab64960)
Key features and details
- Rabbit polyclonal to p35
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-p35 antibody
See all p35 primary antibodies -
Description
Rabbit polyclonal to p35 -
Host species
Rabbit -
Specificity
Detects endogenous levels of total p35 protein -
Tested Applications & Species
Application Species ICC/IF HumanWB Rat -
Immunogen
Synthetic peptide (Human) from an internal sequence
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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WB |
Rat
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All applications |
Mouse
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Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
ICC/IF
1/500 - 1/1000. |
Target
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Function
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII. -
Tissue specificity
Brain and neuron specific. -
Sequence similarities
Belongs to the cyclin-dependent kinase 5 activator family. -
Post-translational
modificationsThe p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35. -
Cellular localization
Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites. - Information by UniProt
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Database links
- Entrez Gene: 8851 Human
- Entrez Gene: 12569 Mouse
- Entrez Gene: 116671 Rat
- Omim: 603460 Human
- SwissProt: Q15078 Human
- SwissProt: P61809 Mouse
- SwissProt: P61810 Rat
- Unigene: 500015 Human
see all -
Alternative names
- CD5R1_HUMAN antibody
- CDK5 activator 1 antibody
- CDK5P35 antibody
see all
Images
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All lanes : Anti-p35 antibody (ab64960) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells, using ab64960 at 1/500 dilution. Right hand panel was also treated with immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab64960 has been referenced in 1 publication.
- Bai X et al. CDK5 promotes renal tubulointerstitial fibrosis in diabetic nephropathy via ERK1/2/PPAR? pathway. Oncotarget 7:36510-36528 (2016). PubMed: 27145370