• Product name
  • Description
    Rabbit polyclonal to p35
  • Host species
  • Specificity
    Detects endogenous levels of total p35 protein
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) from an internal sequence

  • Positive control
    • Rat brain cell extract HeLa cells



Our Abpromise guarantee covers the use of ab64960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.


  • Function
    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • Tissue specificity
    Brain and neuron specific.
  • Sequence similarities
    Belongs to the cyclin-dependent kinase 5 activator family.
  • Post-translational
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • Cellular localization
    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD5R1_HUMAN antibody
    • CDK5 activator 1 antibody
    • CDK5P35 antibody
    • CDK5R antibody
    • CDK5R1 antibody
    • Cyclin dependent kinase 5 regulatory subunit 1 antibody
    • Cyclin-dependent kinase 5 activator 1 antibody
    • Cyclin-dependent kinase 5 regulatory subunit 1 antibody
    • NCK 5A antibody
    • Neuronal CDK5 activator antibody
    • p23 antibody
    • p25 antibody
    • p35 antibody
    • Regulatory partner for CDK5 kinase antibody
    • Tau protein kinase II 23 kDa subunit antibody
    • TPKII regulatory subunit antibody
    see all


  • All lanes : Anti-p35 antibody (ab64960) at 1/500 dilution

    Lane 1 : Rat brain cell extract
    Lane 2 : Rat brain cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

  • Immunofluorescence analysis of HeLa cells, using ab64960 at 1/500 dilution. Right hand panel was also treated with immunising peptide.


ab64960 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply. I look forward to seeing your results from the current western blot. Please let me know if there is anything else I can

Thank you for providing the catalogue number of the antibody. The ab64960, anti-p35 antibody, is guaranteed to work in western blot and so is covered under our Abpromise, which is a guarantee that covers our antibodies for 6 months to work in t...

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