Anti-p35 antibody (ab64960)

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Western blot - Anti-p35 antibody (ab64960)
  • Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (ab64960)

Key features and details

  • Rabbit polyclonal to p35
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-p35 antibody
    See all p35 primary antibodies

  • Description

    Rabbit polyclonal to p35

  • Host species

    Rabbit

  • Specificity

    Detects endogenous levels of total p35 protein

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide (Human) from an internal sequence

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Rat
All applications
Mouse
Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/500 - 1/1000.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/500 - 1/1000.

Target

  • Function

    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.

  • Tissue specificity

    Brain and neuron specific.

  • Sequence similarities

    Belongs to the cyclin-dependent kinase 5 activator family.

  • Post-translational
    modifications

    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.

  • Cellular localization

    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD5R1_HUMAN antibody
    • CDK5 activator 1 antibody
    • CDK5P35 antibody
    • CDK5R antibody
    • CDK5R1 antibody
    • Cyclin dependent kinase 5 regulatory subunit 1 antibody
    • Cyclin-dependent kinase 5 activator 1 antibody
    • Cyclin-dependent kinase 5 regulatory subunit 1 antibody
    • NCK 5A antibody
    • Neuronal CDK5 activator antibody
    • p23 antibody
    • p25 antibody
    • p35 antibody
    • Regulatory partner for CDK5 kinase antibody
    • Tau protein kinase II 23 kDa subunit antibody
    • TPKII regulatory subunit antibody
    see all

Images

  • Western blot - Anti-p35 antibody (ab64960)
    Western blot - Anti-p35 antibody (ab64960)
    All lanes : Anti-p35 antibody (ab64960) at 1/500 dilution

    Lane 1 : Rat brain cell extract
    Lane 2 : Rat brain cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (ab64960)
    Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (ab64960)
    Immunofluorescence analysis of HeLa cells, using ab64960 at 1/500 dilution. Right hand panel was also treated with immunising peptide.

References (1)

Publishing research using ab64960? Please let us know so that we can cite the reference in this datasheet.

ab64960 has been referenced in 1 publication.

  • Bai X  et al. CDK5 promotes renal tubulointerstitial fibrosis in diabetic nephropathy via ERK1/2/PPAR? pathway. Oncotarget 7:36510-36528 (2016). PubMed: 27145370

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I ran the Hela/Rat brain blots today and will try various probing conditions tomorrow. If these are positive, I can send you the figures for your own records. Hopefully this will tell us something about the endogenous levels of p35 in HEK cells too… Thanks for your help!

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Answer

Thank you for your reply. I look forward to seeing your results from the current western blot. Please let me know if there is anything else I can

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Question

Thank you for your reply. The catalogue antibody is ab64960 Please let me know if you have some suggestions, Best wishes, Tania

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Answer

Thank you for providing the catalogue number of the antibody. The ab64960, anti-p35 antibody, is guaranteed to work in western blot and so is covered under our Abpromise, which is a guarantee that covers our antibodies for 6 months to work in the applications and species as stated on the data sheet. I was wondering if HEK-293 cells actually express p35, I ask as one of our other p35 antibodies, ab66064, was tested in transfecetd vs non-transfected HEK-293T cells, and no signal for p35 was detected in the non-transfected cells. Have you tried to run HEK-293 cell lysate samples and either HeLa cell or rat brain lysates and see if you get a signal from any of these samples? Please let me know if you have tried any of the tips from the previous email, or whether you have any information as to whether p35 is endogenously expressed in HEK293 cells. If there is anything else I can help with, please do not hesitate to contact

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