Key features and details
- Rabbit polyclonal to p38
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-p38 antibody
See all p38 primary antibodies
DescriptionRabbit polyclonal to p38
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human p38 MAPK. Synthesized non-phosphopeptide derived from around the phosphorylation site of tyrosine 322 (D-P-Y-D-Q).
Database link: Q16539
- ICC/IF: NIH/3T3 cells. IHC-P: Human brain tissue. WB: Extracts from HEK-293 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
All lanes : Anti-p38 antibody (ab197348) at 1/500 dilution
Lane 1 : Extracts from HEK-293 (Human epithelial cell line from embryonic kidney) cells
Lane 2 : Extracts from HEK-293 (Human epithelial cell line from embryonic kidney) cells with synthesized peptide
Predicted band size: 41 kDa
NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for p38 using ab197348 at a dilution of 1/100 in ICC/IF. Right image treated with synthesized peptide.
Paraffin-embedded human brain tissue stained for p38 with ab197348 at a 1:50 dilution in immunohistochemical analysis. Right image treated with synthesized peptide.
ab197348 has been referenced in 25 publications.
- Zhang Y et al. Mycobacterium tuberculosis Heat-Shock Protein 16.3 Induces Macrophage M2 Polarization Through CCRL2/CX3CR1. Inflammation 43:487-506 (2020). PubMed: 31748849
- Jia T et al. MiR-148a inhibits oral squamous cell carcinoma progression through ERK/MAPK pathway via targeting IGF-IR. Biosci Rep 40:N/A (2020). PubMed: 32202300
- Lyu X et al. Ginsenoside Rh1 inhibits colorectal cancer cell migration and invasion in vitro and tumor growth in vivo. Oncol Lett 18:4160-4166 (2019). PubMed: 31579419
- Yu MH et al. SAA1 increases NOX4/ROS production to promote LPS-induced inflammation in vascular smooth muscle cells through activating p38MAPK/NF-?B pathway. BMC Mol Cell Biol 20:15 (2019). PubMed: 31216990
- Geng W & Liu L MiR-494 alleviates lipopolysaccharide (LPS)-induced autophagy and apoptosis in PC-12 cells by targeting IL-13. Adv Clin Exp Med 28:85-94 (2019). PubMed: 30170481