Overview

  • Product name

  • Description

    Rabbit polyclonal to p38
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human p38 MAPK. Synthesized non-phosphopeptide derived from around the phosphorylation site of tyrosine 322 (D-P-Y-D-Q).
    Database link: Q16539

  • Positive control

    • ICC/IF: NIH/3T3 cells. IHC-P: Human brain tissue. WB: Extracts from HEK-293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab197348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificity

    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    • Stress-activated protein kinase 2a antibody
    see all

Images

  • All lanes : Anti-p38 antibody (ab197348) at 1/500 dilution

    Lane 1 : Extracts from HEK-293 (Human epithelial cell line from embryonic kidney) cells
    Lane 2 : Extracts from HEK-293 (Human epithelial cell line from embryonic kidney) cells with synthesized peptide

    Predicted band size: 41 kDa

  • NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for p38 using ab197348 at a dilution of 1/100 in ICC/IF. Right image treated with synthesized peptide.

  • Paraffin-embedded human brain tissue stained for p38 with ab197348 at a 1:50 dilution in immunohistochemical analysis. Right image treated with synthesized peptide.

References

This product has been referenced in:

  • Gan L  et al. Long Non-Coding RNA ZEB1-Antisense 1 Affects Cell Migration and Invasion of Cervical Cancer by Regulating Epithelial-Mesenchymal Transition via the p38MAPK Signaling Pathway. Gynecol Obstet Invest 84:136-144 (2019). Read more (PubMed: 30253398) »
  • Pan BL  et al. Decreased microRNA-182-5p helps alendronate promote osteoblast proliferation and differentiation in osteoporosis via the Rap1/MAPK pathway. Biosci Rep 38:N/A (2018). Read more (PubMed: 30413613) »
See all 18 Publications for this product

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