Overview

  • Product name

  • Description

    Rabbit polyclonal to p38
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human p38 conjugated to keyhole limpet haemocyanin (Cysteine residue). 20 amino acids
    Database link: Q16539

  • Positive control

    • WB: A431, A549, HCT 116, HeLa, HEK-293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3, and T98G cell lysates; Rat brain lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).
ICC/IF 1/100.

Target

  • Function

    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificity

    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    • Stress-activated protein kinase 2a antibody
    see all

Images

  • 2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for p38 (green) using ab223619 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT.

    (A) DAPI nuclear stain (B) ab223619 (C) Merge.

  • All lanes : Anti-p38 antibody (ab223619) at 1/4000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
    Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
    Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 12 : T98G cell lysate
    Lane 13 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG HRP

    Predicted band size: 41 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 1.5% BSA.

    Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.

  • 2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for p38 (green) using ab223619 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. 

    (A) DAPI nuclear stain (B) ab223619 (C) Merge.

References

ab223619 has not yet been referenced specifically in any publications.

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